原子吸收分光光度法测定生物样品中的游离脂肪酸  

Determination of Free Fatty Acid in Organism Samples Using Atomic Absorption Spectrometry

作　　者：刘忠英[1] 胡秀丽[1] 金贞燕[1] 安汝国 

机构地区：[1]白求恩医科大学应用基础医学研究所,长春130021

出　　处：《分析化学》1993年第4期446-448,共3页Chinese Journal of Analytical Chemistry

摘　　要：本文研究了用原子吸收分光光度法间接测定生物样品中游离脂肪酸(FFA)的方法,用氯仿-正己烷-甲醇混合液萃取样品中FFA,用铜溶液与FFA形成“铜皂”,进入有机相,未反应的Cu^(2+)进入水相,用原子吸收法测定有机相中铜,间接求得游离脂肪酸含量。本法线性范围为10～100nmol,对血浆测定结果的相对标准偏差为<6.59％,回收率为96％～102％。本法的测定结果与比色法的结果基本一致。A new method for the determination of free fatty acid (FFA) in organism samples using atomic absorption spectrometry was studied. The FFA was extracted from subcellular organelles with chloroform-n-hexane-methanol. Copper ion was added to the organic phase to form the copper soaps with FFA. FFA was determined indirectly by determining the copper in the organic phase. The assay shows a linear response over the range of 10 to 100 nmol of FFA. The recovery of FFA from plasma is 96%-102% . The relative standard deviation is 6. 59%. The result of the determination of sample by this method is in agreement with that by colorimetric method.

关 键 词：原子吸收 分光光度法 脂肪酸 

分 类 号：O623.61[理学—有机化学]