胆红素的间接极谱测定  

Indirect Determination of Bilirubin by Polarography

作　　者：曾百肇[1] 丁军[1] 周性尧[1] 

机构地区：[1]武汉大学化学系,武汉430072

出　　处：《分析化学》1993年第6期621-624,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金

摘　　要：在Hg^(2+)(或Hg^+)-EDTA-磷酸钠缓冲溶液中,胆红素可被迅速氧化成浅绿色产物。该产物可在-0.83V(vs.SCE)左右产生一灵敏的极谱波,其导数波高与胆红素浓度在4×10^(-7)～6×10^(-6)mol/L范围内呈线性关系,因此该波可用于胆红素的间接测试。本文对胆红素的氧化机制和极谱波的性质进行了研究。发现胆红素被氧化成了胆绿素,而所产生的极谱波为一催化波。In Hg(Ⅱ)-EDTA-sodium phosphate buffer solution(pH 11. 5) .bilirubin can be rapidly oxidized to blue product which can produce a stable and sensitive polarographic wave at about-0. 83 V(vs. SCE). The peak height of the wave is linear with bilirubin concentration over the range of 4×10-7 to 6×10-6 mol/L , therefore it can be used indirectly to determine trace amount of bilirubin. In this paper, the blue product is confirmed to be biliverdin and the electrochemical process is considered to contain a catalytic reaction mechanism.

关 键 词：胆红素 极谱 胆绿素 

分 类 号：TQ464.54[化学工程—制药化工]