胆红素的间接光度测定  

Indirect Determination of Bilirubin by Spectrophotometry

作　　者：郑玉霞[1] 曾百肇[1] 王升富[1] 周性尧[1] 

机构地区：[1]武汉大学化学系,武汉430072

出　　处：《分析化学》1993年第10期1159-1161,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金

摘　　要：本文研究了在pH10的氨-氯化铵溶液中铜对胆红素的催化氧化作用及其氧化产物胆绿素与铜形成络合物的性质。该络合物在340nm波长处有最大吸收,可用于对胆红素的间接光度测定。其线性范围为1.2×10^(-6)～3.0×10^(-5)mol/L。In Cu2+-NH3-NH4Cl medium, bilirubin can be rapidly oxidized to biliverdin. The biliverdin can interact with Cu2+ to form a blue complex exhibiting a sensitive absorption peak at about 340 nm. The peak height is linear to bilirubin concentration over a range of 1. 2×10-6 to 3. 0×10-5 mol/L. Therefore the procedure can be used to determine bilirubin. In this paper, the oxidation mechanism of bilirubin and property of the complex are also discussed.

关 键 词：胆红素 胆绿素 络合物 分光光度法 

分 类 号：Q593.1[生物学—生物化学]