毛细管等电聚焦-毛细管区带电泳二维蛋白质分离平台的构建及其性能  

Construction and Performance of Two-dimensional Separation Platform of Coupling Capillary Isoelectric Focusing-zone Electrophoresis for Proteins

作　　者：刘和春[1] 杨春[1] 张维冰[1] 张玉奎[1] 

机构地区：[1]中国科学院大连化学物理研究所,国家色谱研究分析中心大连116011

出　　处：《高等学校化学学报》2004年第8期1451-1453,M010,共4页Chemical Journal of Chinese Universities

基　　金：国家重点基础研究发展计划项目(批准号 :0 0 1CB5 10 2 0 2 )资助

摘　　要：A two-dimensional separation platform of capillary isoelectric focusing(CIEF)-capillary zone electrophoresis(CZE) for proteins was constructed by a microdialysis hollow fiber interface. The interface provided ease of fabrication,speed of mass delivering and convenience of column switching. Chrome c was selected to demonstrate the 2D separation systems feasibility and resolving performance. The 2D separation system was found to greatly increase the resolving power and overall peak capacity over those obtained for either dimension alone.A two-dimensional separation platform of capillary isoelectric focusing(CIEF)-capillary zone electrophoresis(CZE) for proteins was constructed by a microdialysis hollow fiber interface. The interface provided ease of fabrication,speed of mass delivering and convenience of column switching. Chrome c was selected to demonstrate the 2D separation systems feasibility and resolving performance. The 2D separation system was found to greatly increase the resolving power and overall peak capacity over those obtained for either dimension alone.

关 键 词：微透析中空纤维膜接口 二维分离平台 毛细管等电聚焦 毛细管区带电泳 

分 类 号：O658[理学—分析化学]