5-羟色胺敏感膜电极的研究——Ⅰ.离子缔合型5-羟色胺膜电极的研制  

Study on 5-Hydroxytryptamine Sensitive Membrane Electrode Ⅰ. Preparation of Coated-carbon 5-Hydroxytryptamine Membrane Electrode Based on Ion-associating Complexes

作　　者：施林[1] 吕太平[1] 邹波[1] 尹华熙[1] 尹光华[1] 

机构地区：[1]华西医科大学药学院,成都610044

出　　处：《分析化学》1993年第12期1466-1469,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金

摘　　要：5-羟色胺(5-HT)与许多疾病的发生和发展有关。应用正交试验,研究了离子缔合型5-HT敏感膜电极的最优化膜组成。筛选了由3种定域体,7种增塑剂组成的213个配方,选定了以四苯硼-5-HT离子缔合物为活性物、磷酸三(2-乙基己基)酯为增塑剂的涂碳PVC膜电极。电极各项性能良好,其线性范围为1.00×10^(-2)～1.58×10^(-5)mol/L,检测下限为4.17×10^(-6)mol/L,斜率为59.7mV/p5-HT。电极寿命超过39天,用混合溶液法测定了体液中可能存在的17种物质的选择性系数。已用该电极测定了牛血小板中的5-HT。A coated-carbon 5-hydroxytryptamine (5-HT) membrane electrode consisting of sodium te-traphenyl borate-5-HT ion-associated as an active substance,tri(2-ethylhexyl)phosphate as a plasticizer is investigated. The general performance characteristics of the electrode are good. The linear response of the electrode is over the concentration range of 1. 00×10 2-1. 58×10 5mol/L for serotonin creatinine sulfate. The detection limit is 4. 17×10 6mol/L, and slope is 59. 7 mV/p5-HT. The lifetime is over 39 d. Selectivity coefficients of 17 substances were determined by the mixed solution method. 5-HT in bull platelet was determined by using the electrode.

关 键 词：血清素 敏感膜电极 四苯硼钠 电极 

分 类 号：O646.5[理学—物理化学]