火焰原子吸收光谱法测定红细胞膜结合镁  

Flame Atomic Absorption Spectrometry Determination of Binding Magnesium in Human Erythrocyte Membrane

作　　者：杨根元[1] 徐德选[1] 何亚楠[1] 邢光伟[1] 陆德澄[2] 苏子杰[2] 凌玲[2] 陈二南[2] 

机构地区：[1]镇江医学院仪器分析教研室,镇江212001 [2]镇江医学院附属医院心血管科

出　　处：《分析试验室》1993年第2期37-39,共3页Chinese Journal of Analysis Laboratory

摘　　要：本文用火焰原子吸收光谱法测定红细胞膜结合镁含量。测定健康者45人,膜结合镁平均值为1.15±0.065μg/mg膜蛋白;原发性高血压患者26人,膜结合镁平均值为0.91±0.028μg/mg膜蛋白;冠心病患者30人,膜结合镁平均值为0.90±0.039μg/mg膜蛋白。由测定结果表明,原发性高血压组和冠心病组胞膜结合镁含量明显低于对照组概率(p<0.05)。该方法的回收率为98.28～104.54%,相对标准偏差小于3.90%。The present study was performed to determine the amount of binding magnesium in human erythrocyte membrane by using flame AAS. In forty-five healthy subjects, the average of binding magnesium in erythocyte membrane was 1.15～0.065μg/mg membrane proteins; in twenty-six patients with primary hypertension, the average of binding magnesium in red erythrocyte membrane was 0.91±0.028μg/mg membrane proteins; in thirty cases with coronary heart disease, the average of binding magnesium erythrocyte membrane was 0.90±0.039μg/mg membrane proteins. The difference was significant (p <0.05). As to the determination method, the rate of recovery varied from 98.28% to 104.54% and the RSD was less than 3. 9%.

关 键 词：原子吸收光谱 红细胞膜 镁 

分 类 号：Q241[生物学—细胞生物学]