钕对白沙枇杷试管苗氮代谢的效应研究  

Effects of Neodymium on Nitrogen Metabolisim of Plantlet Loquat in vitro

作　　者：万志刚[1] 宋卫平[1] 洪法水[1] 俞明亮[2] 常有宏[2] 顾福根[1] 苏国兴[1] 

机构地区：[1]苏州大学生命科学学院,江苏苏州215006 [2]江苏省农业科学院园艺所,江苏南京210049

出　　处：《中国稀土学报》2004年第3期380-384,共5页Journal of the Chinese Society of Rare Earths

基　　金：江苏省自然科学基金项目 (BK2 0 0 2 5 0 1)

摘　　要：将不同浓度的NdCl3加入白沙枇杷组培苗生根培养基中 ,研究了NdCl3对白沙枇杷试管苗氮代谢的影响。结果表明 ,白沙枇杷生根培养基 1/ 2MS +IBA 2 .5 μmol·L- 1 中加入 0 .3～ 0 .5 μmol·L- 1 NdCl3可显著提高其生根率、促进根的伸长生长和根重量的增加 ,增强根系和叶片硝酸还原酶、谷氨酰胺合成酶及谷氨酸脱氢酶的活性 ,促进硝态氮转变成氨态氮和蛋白质 ,加快氮代谢的速度。To study the effect of NdCl_3 on the rooting of loquat plantlet in vitro, NdCl_3 was added to the rooted medium. The rooting rate, the number of root and the length of root were studied after transplanting 15 d. After transplanting 42 d, the activity of nitrate reductase, glutamine syntheta, glutamine dehydrogenase, the content of NO_3^-, NH_4^+, protein and fresh weight of roots were determined. The result shows that the optimum concentration of Nd^(3+) in the range of 0.3～0.5 μmol·L^(-1) in the rooted medium increases the rooting rate, the fresh weight of roots, and enlarges the length of root. The activities of nitrate reductase, glutamine syntheta, glutamine dehydrogenase in the roots and leaves increase significantly, and the content of NO_3^-, NH_4^+ decreases and the protein content raises.

关 键 词：农业生物学 钕 白沙枇杷 组培苗 生根培养 氮代谢 稀土 

分 类 号：Q944[生物学—植物学]