近红外光谱法同时测定银杏提取液中总黄酮和总内酯含量  

Simultaneous Determination of Total Flavones and Total Lactones in Ginkgo Extracts by Near Infrared Spectroscopy

作　　者：胡钢亮[1] 吕秀阳[1] 罗玲[1] 徐铸德[2] 

机构地区：[1]浙江大学制药工程研究所,杭州310027 [2]浙江大学化学系,杭州310027

出　　处：《分析化学》2004年第8期1061-1063,共3页Chinese Journal of Analytical Chemistry

基　　金：教育部优秀青年教师资助计划 (教人司 [2 0 0 2 ] 40 );高等学校博士学科点专项科研基金 (No .2 0 0 10 3 3 5 0 2 9)资助项目

摘　　要：采用傅里叶变换近红外透射光谱和偏最小二乘法 ,对银杏提取液中总黄酮和总内酯的含量进行了定量测定。所建立的数学模型对校正集样本的银杏总黄酮和总内酯复相关系数 (MR)分别为 0 .998和 0 986 ;对预测集样本的银杏总黄酮和总内酯复相关系数分别为 0 .983和 0 .971 ,标准回收率分别为 95 71 %～ 1 0 3.3%和95 2 9%～ 1 0 4 .6 % ,相对标准偏差 (RSD)分别为 2 .4 4 %和 3.1 9%。结果表明 ,近红外光谱测定银杏提取液中总黄酮和总内酯含量具有快速、准确的优点 。A new method was established to simultaneously determine the total flavones and total lactones in ginkgo extracts by Fourier transform near infrared spectroscopy (FT-NIR) with partial least square (PLS). The multicorrelahon coefficients (MR) of the model to the total flavones and total lactones of the calibration samples were 0.998 and 0.986 respectively. The multicorrelation coefficients of the model to the total flavones and total lactones of the prediction samples were 0.985 and 0.971, the recoveries were 95.7%-103.3%, and 95.3%-104.6%, the RSDs were 2.4% and 3.2%. The result shows that the method proposed can be used to simultaneously determine the total flavones and total lactones in ginkgo extract with high accuracy.

关 键 词：银杏提取液 总黄酮 总内酯 含量测定 近红外光谱法 同时测定 

分 类 号：O657.33[理学—分析化学]