碱性二苯基萘基甲烷染料褪色光度法测定透明质酸钠  

Fading Spectrophotometric Method for the Determination of Sodium Hyaluronate with Basic Bisphenylnaphthylmethane Dyes

作　　者：陈莲惠[1] 刘绍璞[1] 罗红群[1] 胡小莉[1] 

机构地区：[1]西南师范大学化学化工学院

出　　处：《分析化学》2004年第8期1086-1089,共4页Chinese Journal of Analytical Chemistry

基　　金：重庆市科委资助项目 (No .2 0 0 2 7472 );四川省教育厅青年科学基金资助项目 (No .2 0 0 3B0 13 )

摘　　要：在弱酸性介质中 ,碱性二苯基萘基甲烷染料与透明质酸钠作用形成结合产物时将导致溶液光吸收发生变化 ,在 5 85～ 6 1 6nm附近褪色且吸光度差 (ΔA)值与透明质酸钠浓度成正比 ,在 0 .1～ 2 .5mg/L(维多利亚蓝B)、0 .5～ 2 .0mg/L(夜蓝 )和 0 .1～ 0 .6mg/L(维多利亚蓝 4R)范围内符合Beer定律 ,表观摩尔吸光系数达 1 .5 1× 1 0 8～ 5 .5 2× 1 0 8L·mol-1 ·cm-1 (透明质酸钠的分子量以 5× 1 0 5计 ) ,其中 ,维多利亚蓝 4R灵敏度最高。据此 ,建立了一种新的测定透明质酸钠的分光光度法。该法简便、快速 ,灵敏度高 ,选择性好。应用于润舒氯霉素滴眼液、润洁滴眼露和施沛特注射液中透明质酸钠的测定 。In a weak acid medium, bisphenynaphthylmethane dyes such as night blue (NB) Victoria blue B (VBB) and Victoria blue 4R (VB4R), react rapidly with sodium hyaluronate to form complexes, which cause the change of absorption spectra, the decrease of absorbance near 612 nm(NB), 616 nm(VBB), 585 nm(VB4R), separately, and DeltaA(A-A(0)) values are proportional to the concentration of sodium hyaluronate, Beer's law is obeyed in the range between 0.5similar to2.0 mg/L(NB), 0.1similar to2.5 mg/L(VBB) and 0.1similar to0.6 mg/L (VB4R) respectively for sodium hyaluronate. The molar absorptivities of different systems are between 1.51x10(8)similar to5.52x10(8) L.mol(-1).cm(-1)(the molecule of SH is 5x10(5)). Based on these, we developed a new spectrophotometric method for the determination of sodium hyaluronate. The method is simple, rapid, repeatable, and of good selectivity, and has been used for the determination of total amounts of sodium hyaluronate in samples with satisfactory results.

关 键 词：碱性二苯基萘基甲烷染料 褪色光度法 透明质酸钠 滴眼液 注射液 含量测定 

分 类 号：O657.3[理学—分析化学]