^(60)Coγ射线辐照花魔芋球茎的早期诱变效应研究  

Study on Early Effects of Amorphophallus konjac Irradiated by ^(60)Coγ Ray

作　　者：黄训端[1] 周立人[1] 何家庆[2] 徐明照[1] 

机构地区：[1]安徽农业大学生命科学学院,中国安徽合肥230036 [2]安徽大学生命科学学院,中国安徽合肥230039

出　　处：《激光生物学报》2004年第4期306-313,共8页Acta Laser Biology Sinica

基　　金：科技部攻关课题 (99 0 1 0 0 1 1 3);安徽省自然科学基金项目

摘　　要：本试验开展了花魔芋早期诱变效应研究。通过6 0 Coγ射线辐照后的植株苗期发育观察和根尖细胞学检测分析 ,结果表明魔芋辐射诱变效应显著 :辐照诱发核畸变和染色体畸变 ,且变异频率与剂量呈二次曲线关系 ;低剂量时对细胞分裂有刺激作用 ;辐照抑制芽体发育、苗期生长 ,抑制效应随剂量增加而加大 ,直至产生致死作用。依据花魔芋的辐射敏感性 ,建立了魔芋辐照诱变体系 ,以催芽球茎为诱变材料 ,诱变剂量范围为 0Gy～ 5 0Gy,剂量率为1Gy min ,中等适宜剂量为 7Gy～ 1 0Gy,致死剂量 5 0Gy。The experiment was designed to study early effects of Amorphophallus Konjac irradiated by ()^(60)Coγ ray.As a result,the evident early induced effects of Konjac were obtained.Nucleus aberration and chromosome aberration of Konjac root tip cell were tested,and their frequencies change as conic with dose.The stimulating effects were found in lower dose.Yet irradiation restrains growth of bud and seedling,and the effects are increasing with dose up,till death with lethal dose.A system of irradiation has been established according to the Konjac irradiation sensitivity.The system contains irradiation materials: Konjac hastening germination corm, dose range: from 0Gy to 50Gy,dose rate:1Gy/min, middling optimum dose range: from 7Gy to 10Gy,lethal dose:50Gy.

关 键 词：^60Coγ射线 花魔芋球茎 诱变效应 

分 类 号：S632.9[农业科学—蔬菜学]