青蒿鞣质抗病毒活性研究  

STUDY ON THE ANTIVIRAL ACTIVITIES OF CONDENSED TANNIN OF ARTEMISIA ANNUA L.

作　　者：张军峰[1] 谭健[1] 蒲蔷[1] 刘颖华[1] 刘月雪[1] 何开泽[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041

出　　处：《天然产物研究与开发》2004年第4期307-311,共5页Natural Product Research and Development

摘　　要：从青蒿水提物中分离得到一种缩合鞣质 (CTA) ,在体外进行了抗HSV 2和HBV活性研究。结果表明 ,CTA具有显著抗HSV 2活性 ,与阳性对照药物阿昔洛韦 (ACV)比较 ,CC50 分别为 6 .84mg/mL和 3.6 9mg/mL ,IC50 分别为 0 .16 2mg/mL和 0 .138mg/mL ,表明CTA的细胞毒性比ACV小 ,最低抗病毒活性浓度相当。用含CTA的培养基培养HepG2 .2 .1.5细胞 ,结果表明在浓度为 2 .5mg/mL时有轻微细胞毒性 ,在浓度为 2 .5～ 0 .15 6mg/mL对HepG2 .2 .1.5细胞表达的HBeAg具有显著的抑制作用 ,0 .6 2 5mg/mL浓度档培养第 12d对HBeAg抑制率达到 90 .4 5 % 。Inhibitory effects of condensed tannin of Artemisia annua L.(CTA) on herpes simplex virus type 2(HSV-2) and HBeAg secretion in cultured HepG2.2.1.5 cell line were investigated.When the anti-HSV-2 activity of CTA was tested with acyclovir(ACV) as contrast drug,CC 50 of CTA and ACV were 6.84 mg/mL and 3.69 mg/mL,IC 50 were 0.162 mg/mL and 0.138 mg/mL respectively.The results showed CTA was as effective as the clinical drug-ACV.To study the anti-hepatitis B virus activity of CTA,the cytotoxicity to 2.2.1.5 cell line and inhibition of HBeAg secretion were tested.The results showed that CTA had slight cytotoxicity at 2.5 mg/mL and can distinctly inhibit the secretion of HBeAg in HepG2.2.1.5 cell line.All the results indicated that CTA maybe have a high selectivity index against HSV and HBV.

关 键 词：缩合鞣质 细胞毒性 CTA 阿昔洛韦 抗病毒活性 HBV 乙型肝炎病毒 

分 类 号：R285[医药卫生—中药学] R373.21[医药卫生—中医学]