RP-HPLC法测定盾叶薯蓣细胞中薯蓣皂甙元的含量  

DETERMINATION OF DIOSGENIN IN DIOSCOREO ZINGIBERENSIS CELL BY RP-HPLC

作　　者：顾永明[1] 袁丽红[1] 李文谦[1] 欧阳平凯[1] 

机构地区：[1]南京工业大学制药与生命科学学院,南京210009

出　　处：《天然产物研究与开发》2004年第4期331-333,共3页Natural Product Research and Development

基　　金：国家自然科学基金项目 ( 2 0 2 760 3 0 )

摘　　要：本文采用KromasilC - 18柱 (2 5 0× 4 6mm ,5 μm ) ,甲醇∶水 =95∶5流动相 ,流速 1 0mL/min ,UV检测器 ,检测波长 2 0 8nm ,对培养的盾叶薯蓣细胞中的薯蓣皂甙元含量进行测定 ,能将薯蓣皂甙元与杂质分离开 ,薯蓣皂甙元在 3 876～ 19 38μg范围内峰面积与其浓度线性关系良好 (R =0 9993) ,样品平均回收率为97 83% ,精密度RSD为 0 2 1% (n =4 )。该法测定样品快速准确 。A method for the separation and determination of diosgenin in cultivated Dioscorea cell by reversed-phase high performance liquid chromatography was developed?The operating conditions were Kromasil C-18 column(250×4.6 mm,5 μm),mobile phase methylalcohol∶water=95∶5 1.0 mL/min as flow and UV detector at 208 nm? The linearity of the calibration curve was good in the range of 3.876～19.38 μg for diosgenin(R=0.9993)?The average recovery and RSD of diosgenin were 97.83% and 0.21%(n=4) respectively?The method is accurate and reproducible to determine the sample.

关 键 词：盾叶薯蓣 细胞 薯蓣皂甙元 反相高效液相色谱 RP-HPLC 黄姜 百合目 

分 类 号：Q949.718.2[生物学—植物学]