利用有序差异显示技术克隆受稻瘟病菌诱导表达的水稻cDNA的研究  

Application of Ordered Differential Display to Isolate Rice cDNAs Induced by Magnaporthe grisea

作　　者：罗文永[1] 李晓方[2] 胡骏[1] 刘文华[1] 肖昕[2] 刘良式[1] 

机构地区：[1]中山大学植物基因工程教育部重点实验室,广州510275 [2]广东省农业科学院水稻研究所

出　　处：《生物化学与生物物理进展》2004年第7期635-642,共8页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目 ( 3 0 0 40 0 2 5 );广东省科技攻关项目 ( 99M0 15 0 3G)~~

摘　　要：利用有序差异显示技术 (ODD)分析受稻瘟病菌诱导表达的水稻基因 ,通过反式RNA印迹进行辅助筛选 ,获得了 37个在接种稻瘟病菌后表达量增强的水稻cDNA克隆 .采用RNA印迹对其中 5个克隆在接种稻瘟病菌后的表达分析表明 ,这些克隆在抗病以及感病的水稻株系中都具有诱导表达的特点 .根据序列同源性分析 ,与这些克隆序列相应的同源基因可能涉及抑制病原菌生长、清除真菌毒素、传递抗病信号以及调节宿主生理状态等几方面的功能 .Ordered differential display (ODD) was used to clone differentially expressed genes in rice induced by Magnaporthe grisea. Of 37 cDNA clones initially identified by reverse Northern blot, 5 clones were chosen as probes for further Northern test. The result showed that all of these 5 clones were expressed both in highly, moderately resistant and susceptible rice lines in a pathogen inducible manner. According to the homology analysis, these clones were suggested to be involved in plant defense response by: ( I) inhibiting growth of fungus and even causing its death; (2) excluding toxic compounds delivered by fungus; (3) priming signal transduction; (4) and/or regulating metabolic activities in agreement with plant defense.

关 键 词：有序差异显示技术(ODD) 水稻 稻瘟病菌 CDNA 克隆 

分 类 号：Q943[生物学—植物学]