Impaired reproduction in transgenic mice overexpressing γ-aminobutyric acid transporter Ⅰ (GAT1)  

Impaired reproduction in transgenic mice overexpressing γ-aminobutyric acid transporter I （GAT1）

作　　者：JiaHuaHU JinFuZHANG YingHuaMA JieJIANG NaYANG XinBoLI ZhiGuangYUCHI JianFEI LiHeGUO 

机构地区：[1]LaboratoryofMolecularCellBiology,InstituteofBiochemistryandCellBiology,ShanghaiInstitutesforBiologicalSciences,ChineseAcademyofSciences,Shanghai200031,China. [2]DepartmentofUrology,TongjiHospital,TongjiUniversity,389XincunRoad,Shanghai200065,China. [3]NewDrugR＆DCenter,NorthChinaPharmaceuticalGroupCorporation,388HepingEastRoad,ShijiaZhuang050015,Hebei,China. [4]Schooloflifesciences,FudanUniversity,220HandanRoad,Shanghai200433,China. [5]ShanghaiResearchCenterforBiomodelOrganism,Shanghai201203,China.

出　　处：《Cell Research》2004年第1期54-59,共6页细胞研究（英文版）

基　　金：supported by foundations from Chinese Academy of Sciences;Special Funds for Major State Basic Research Development program of China(G19990539).

摘　　要：It is well documented that γ-aminobutyric acid (GABA) system existed in reproductive organs. Recent researches showed that GABA_A and GABA_B receptors were present in testis and sperm, and might mediate the acrosome reaction induced by GABA and progesterone. GABA transporter Ⅰ (GAT1) also existed in testis and sperm, but its physiological function was unknown. In the present study, we used GAT1 overexpressing mice to explore GAT1 function in male reproductive system. We found that the expression level of GAT1 continuously increased in wild-type mouse testis from 1 month to 2 months after birth. GAT1 overexpression in mouse affected testis development, which embodied reduced testis mass and slowed spermatogenesis in transgenic mice. Moreover, transgenic mice showed increase of the percentage of broken sperm. The further study revealed that the reproductive capacity was impaired in GAT1 overexpressing mice. In addition, testosterone level was significantly low in transgenic mice compared with that in wild-type mice. Our findings provided the first evidence that abnormal expression of GAT1 could result in dysgenesis, and indicated that GAT1 might be therapeutically targeted for contraception or dysgenesis treatment.

关 键 词：GABA GAT1 TESTES SPERM REPRODUCTION transgenic. 

分 类 号：Q959.837[生物学—动物学] Q78