两种鱼类Sox基因保守区的克隆和Sox9基因保守区内含子的遗传变异性  

Sequencing of Sox gene HMG-box and intron genetic variability of Sox9 gene in conservative HMG-box in two fishes

作　　者：陈金平[1] 孙大江[2] 梁冰[1] 石兴娣[3] 吴文化[2] 张树义[1] 

机构地区：[1]中国科学院动物研究所,北京100080 [2]中国水产科学研究院鲟鱼繁育技术工程中心,北京100039 [3]北京师范大学生命科学学院,北京100875

出　　处：《动物学报》2004年第4期681-685,共5页ACTA ZOOLOGICA SINICA

基　　金：中国科学院知识创新工程重要创新方向项目 (KSCX2 SW 118);国家自然科学基金杰出青年基金项目 (No . 3 0 0 2 5 0 0 7)~~

摘　　要：Four different Sox genes in Amur sturgeon Acipenser schrenckii and five Sox genes in chum salmon Oncorhynchus keta were identified for the first time in this paper, and there was no sexual difference for these genes in these two fishes. For Amur sturgeon, the encoded amino acid sequences exhibited 98%, 98%, 98%, 86% of sequence homology with those reported for human SOX HMG box, respectively, and for chum salmon, the encoded amino acid sequences exhibited 93%,98%,98%,98%,96% of sequence homology with those reported for human SOX HMG box, respectively. Two different forms of Sox9 gene in chum salmon were found, and Sox9 contained a 303 bp and 258 bp introns in the HMG box for chum salmon respectively while 572 bp for Amur sturgeon. No clear similarity was observed between the introns in the Sox9 HMG box of different fishes, but the introns occur in the HMG box region between the second and third bases of the codon for arginine at residue 42 in HMG box for the three fishes, and obey the rule of “GT AG”. and the encoded amino acid sequences in the three Sox9 genes HMG box are very conservative in the two fishes (100% identification). Identification of these genes is a potential step in understanding development regulations including sex determination in Amur sturgeon.Four different Sox genes in Amur sturgeon Acipenser schrenckii and five Sox genes in chum salmon Oncorhynchus keta were identified for the first time in this paper, and there was no sexual difference for these genes in these two fishes. For Amur sturgeon, the encoded amino acid sequences exhibited 98%, 98%, 98%, 86% of sequence homology with those reported for human SOX HMG box, respectively, and for chum salmon, the encoded amino acid sequences exhibited 93%,98%,98%,98%,96% of sequence homology with those reported for human SOX HMG box, respectively. Two different forms of Sox9 gene in chum salmon were found, and Sox9 contained a 303 bp and 258 bp introns in the HMG box for chum salmon respectively while 572 bp for Amur sturgeon. No clear similarity was observed between the introns in the Sox9 HMG box of different fishes, but the introns occur in the HMG box region between the second and third bases of the codon for arginine at residue 42 in HMG box for the three fishes, and obey the rule of “GT AG”. and the encoded amino acid sequences in the three Sox9 genes HMG box are very conservative in the two fishes (100% identification). Identification of these genes is a potential step in understanding development regulations including sex determination in Amur sturgeon.

关 键 词：史氏鲟 大麻哈鱼 SOX基因 克隆 外显子 内含子 遗传变异性 

分 类 号：Q786[生物学—分子生物学]