冬小麦丙二烯氧化合酶基因(TaAOS)的克隆及其特性(英文)  

Cloning and Characteristics of anAllene Oxide Synthase Gene (TaAOS) of Winter Wheat

作　　者：吴劲松[1] 种康[1] 徐云远[1] 谭克辉[1] 

机构地区：[1]中国科学院植物研究所光合及环境分子生理重点实验室分子与发育生物学研究中心,北京100093

出　　处：《植物生理与分子生物学学报》2004年第4期413-420,共8页Journal Of Plant Physiology and Molecular Biology

基　　金：中国科学院知识创新工程项目;国家高技术研究发展计划项目(2001AA222281)资助。~~

摘　　要：丙二烯氧化合酶(allene oxide synthase, AOS)是茉莉酸脂加氧酶合成途径过程中的第一个酶。从冬小麦(Triticum aestivum L. cv. Jinghua No.3)克隆到了该酶的一个全长 cDNA 片段,其开放阅读框长约 1 410 bp,编码一约含470个氨基酸残基的多肽,推测其分子量为 51.9 kD。Southern 分析显示其在基因组中以 3 个拷贝的形式存在。Northern杂交分析表明该基因表达可被外源的茉莉酸诱导,诱导10 h时达到高峰,进一步的RNA 原位杂交表明该基因优先在幼叶中,尤其是在维管束附近的薄壁细胞中表达。同时,原位杂交还显示质膜钙通道的抑制剂 La3+并不能抑制外源茉莉酸诱导该区域 TaAOS 的表达。Allene oxide synthase (AOS) is the first enzyme in the lipoxygenase pathway which leads to the formation of jasmonic acid (JA). A full length cDNA of TaAOS was cloned in winter wheat (Triticum aestivum L. cv. Jinghua No.3) seedlings. The open reading frame encompassed 1 410 bp encoding a polypeptide of 470 amino acids with calculated mo- lecular mass of 51.9 kD. Southern blot analysis sug- gested there are three copies of the gene in wheat genome. The TaAOS mRNA could be strongly induced by exogenous JA, and the highest level JA was ob- served after a 10 h induction. In situ RNA hybridiza- tion of seedling indicated preferential gene expression in young leaves, especially in the parenchyma cells around the vascular bundles, and the hybridization also showed that exogenous La3+ could not suppress the expression of TaAOS induced by JA.

关 键 词：冬小麦 丙二烯氧化合酶基因(TαAOS) 基因表达 茉莉酸 小麦 

分 类 号：S512.11[农业科学—作物学]