具有间充质干细胞特征的CD105^+细胞在胎儿多种组织器官的分布  

The Distribution of CD105^+ Cells with Characteristics of Mesenchymal Stem Cells in Diverse Fetal Tissues and Organs

作　　者：郭虹[1] 刘杰文[1] 杨少光[1] 曹德骏 刘津华[1] 廖联明[1] 赵春华[1] 

机构地区：[1]中国医学科学院,中国协和医科大学血液学研究所,组织工程中心,实验血液学国家重点实验室,天津300020

出　　处：《细胞生物学杂志》2004年第4期404-408,共5页Chinese Journal of Cell Biology

基　　金：国家高技术研究发展计划资助项目(863计划)(No.2002AA205061)ChinaMedicalBoardofNewYork;Inc(Grant#01-748)资助项目~~

摘　　要：CD105是骨髓间充质干细胞的特征性表型之一。为了研究机体各组织器官也存留有间充质干细胞,首先检测胎儿各组织CD105+细胞的分布,进而分离胎儿各组织CD105+细胞。将CD105+细胞向脂肪和成骨细胞诱导分化。结果表明胎儿心、肝、肺、血管、肌肉、皮肤等组织含有CD105+间充质干细胞。在间充质干细胞分化为脂肪细胞时,CD105表达明显下降。地塞米松可以促进脂肪细胞形成并提高了培养液中甘油三酯的含量。而向成骨细胞分化时,诱导的成骨细胞胞浆内外有电子密度高的钙盐沉积。以上结果提示,分布于多种组织的间充质干细胞异常分化可能与疾病的发生有关。To investigate whether there are CD105+ mesenchymal stem cells (MSCs) in fetal diverse tissues and organs besides MSCs derived from bone marrow, we assessed the distribution of CD105 in fetal diverse tissues and organs, and isolated CD105+ cells from various tissues. CD105+ cells were induced to differentiate into adipocytes and osteoblasts. The results showed that CD 105+ cells distributed in vascular and mesenchymal cells of heart, liver, spleen, lung, kidney, thymus gland, pancreas, blood vessel, skeletal muscles and derma. The sorted CD105+ cells could differentiate into adipocytes and osteoblasts. During differentiation of MSCs into adipocytes, CD105 expression decreased, strongly supporting that CD105 was a marker of MSCs. Dexamethasone could enhance adipogenesis and the production of triglycerides in the medium of differentiation into adipocytes. During osteogenesis, some needle-shaped crystal calcium deposition similar to bone spicules was also observed both inside and outside of the cytoplasm. These results demonstrated that CD 105+ MSCs distributed in various fetal tissues. The increase of content of triglycerides in the medium of differentiation into adipocytes and calcium deposition inside the cytoplasm indicated CD 105+ MSCs reside in diverse tissues may be related with some pathologic progress.

关 键 词：胎儿 间充质干细胞 脂肪细胞 成骨细胞 分化 CD105 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]