糖尿病大鼠早期肾病模型的实验研究  

Study of Animal Model with Early Diabetic Nephropathy

作　　者：郑学民[1] 林毅[2] 乔文军[1] 王珲[1] 王英[1] 李敬林[1] 

机构地区：[1]辽宁中医学院附属医院，沈阳110032 [2]沈阳市第一人民医院，110042

出　　处：《中国实验动物学杂志》2002年第5期288-291,共4页Chinese Journal of Laboratory Animal Science

摘　　要：目的　研究糖尿病大鼠早期肾病的中西医造模方法。方法　利用单侧肾切除合并尾静脉注射链脲佐菌素 (STZ)及高脂饮食的方法造成糖尿病早期肾病 (DN)模型及痰浊血瘀中医证候模型。应用自拟的具有祛痰化瘀功效的中药糖克煎剂于造模成功后灌胃治疗 4周 ,以反证此中医证候模型是否成立。实验前后测定大鼠血糖、尿微量白蛋白 (Alb) ,血脂、血流变、肾小球滤过率 (用内生肌酐清除率表示 )。结果　尿微量白蛋白在造模一周后即有明显排出 ,肾小球滤过率、血脂及血流变也有改变。结论　利用单侧肾切除合并尾静脉注射链脲佐菌素及高脂饮食的方法 。Objective To research on producing the rat model of early diabetic nephropathy by traditional Chinese medicine and Western medicine. Methods With one kidney removed, then injection of streptozotocin(STZ) by tail vein and high fat feeding, the model of early diabetic nephropathy and model of traditional Chinese medicine syndrome of both phlegm stagnancy and blood stasis were established, then treated by Tangke in order to prove the models well. A month after treating the urinary albulin(ALB), blood fat, hemodynamics and glomerular filtration rate (GFR) were measured.Results After a week of making models ALB was excluded and GFR also changed. Conclusion The animal models of Chinese medicine and Western medicine are feasible.

关 键 词：糖尿病 大鼠 糖尿病早期肾病 动物模型 代谢紊乱 

分 类 号：R587.1[医药卫生—内分泌] R-332[医药卫生—内科学]