异种动物移植中超急排斥反应动物模型的建立  

Animal Model of Hyperacute Rejection for Intestinal Xenotransplantation

作　　者：王海燕[1] 李涛[1] 郭守利[1] 聂刚[2] 王海霞[1] 林彦[1] 

机构地区：[1]哈尔滨医科大学附属二院动物实验中心,哈尔滨150086 [2]哈尔滨医科大学附属二院普外二科,哈尔滨150086

出　　处：《中国实验动物学杂志》2002年第6期359-361,共3页Chinese Journal of Laboratory Animal Science

摘　　要：目的　用豚鼠对大鼠的异种肠管移植 ,来探索建立异种肠管移植超急排斥反应模型。方法　用豚鼠和Wistar大鼠分别作为供体和受体 ,以豚鼠的远端空肠作为移植物 ,把肠系膜上动脉与主动脉吻合 ,肠系膜上静脉与门静脉吻合 ,移植肠管两端分别与大鼠肠管吻合 ,重建消化道连续性。结果　移植物存活时间为 5min到 4 0min ,超急排斥反应由病理学方法确认。结论　豚鼠到大鼠的小肠移植是肠管异种移植超急排斥反应的一个有效模型 ,该模型的建立有利于进一步探讨异种移植超急排斥反应的免疫学机制 ,并对今后将此技术用于临床提供理论依据。Objective In order to establish the proper animal model of hyperacute rejection(HAR) for xenotransplantation of intestine. Methods Guinea pigs and Wistar rats were used as donors and receptors respectively,distal ileum as grafts.Superior mesenteric vessels were anastomosed to aorta and portal vein.Graft was reconstructed by anastomosis to native guts.Results Survival time of grafts ranged from 5 minutes to 40 minutes, and hyperacute rejection was confirmed by pathology.Conclusion Intestinal xenotransplantation from guinea pig to rat was a suitable model of hyperacute rejection. It would be very useful for the reseach of immune mechanism and for the clinical application.

关 键 词：异种动物 移植 超急排斥反应 动物模型 豚鼠 大鼠 

分 类 号：Q95-331[生物学—动物学] R33-[医药卫生—人体生理学]