普通小麦×大麦杂交后代中间材料的GISH及PAGE鉴定  

Identification of Wheat and Barley Hybrid Offspring by GISH and PAGE

作　　者：陈新宏[1] 王小利[1] 刘淑会[1] 赵继新[1] 武军[1] 李璋[1] 闫正禄[1] 张改生[1] 

机构地区：[1]西北农林科技大学农学院,杨陵712100

出　　处：《Acta Genetica Sinica》2004年第7期730-734,共5页

基　　金：国家"8 63计划"项目 (编号 :2 0 0 1AA2 410 3 7);农业部农业结构调整重大技术研究专项项目 (编号 :2 0 0 2 0 2 0 1A);杨凌农业生物技术育种中心 (编号 :1999 5 )项目资助

摘　　要：利用基因组荧光原位杂交 (GISH)及种子贮藏蛋白聚丙烯酰胺凝胶电泳 (PAGE)对普通小麦×大麦杂交后代中间材料进行了鉴定分析。GISH结果表明 ,WBA984和WBA9812为二体小大麦异附加系 ,WBS0 2 15和WBS0 2 6 4为小大麦二体异代换系 ,WBT0 2 12 5和WBT0 2 183为端部易位系 ;种子贮藏蛋白PAGE分析表明 ,WBA9812和WBS0 2 6 4含有大麦特有的高分子量麦谷蛋白亚基和在γ区含有大麦特有的醇溶蛋白带型 ,WBA9812为大麦 5H附加系 ,WBS0 2 6 4为 1B/ 5H代换系 ,WBT0 2 12 5为 1BL/ 5HL端部易位系。Genomic in situ hybridization (GISH) and polyacrylamide gel electrophoresis (PAGE) of wheat seed were used to identify the barley chromosome in offspring of wheat and barley hybrid.A sires of alien addition lines,alien substitution lines and translocation lines were detected by GISH.WBA984 and WBA9812 were alien addition lines,WBS0215 and WBS0264 were alien substitution lines,and WBT02125 and WBT02183 were translocation lines in chromosome terminal.SDS-PAGE analysis and A-PAGE patterns indicated that WBA9812 were 5H alien addition line,WBS0264 was 1B/5H alien substitution line,and WBT02125 was 1BL/5HL translocation line.

关 键 词：普通小麦 大麦 远缘杂交 GISH PAGE 

分 类 号：S512.1[农业科学—作物学]