大豆灰斑病1号生理小种抗性基因的SSR标记分析  

ANALYSIS OF RESISTANT GENE AGAINST CERCOSPORA SOJINA RACE 1 IN SOYBEAN WITH SSR MARKERS

作　　者：张文慧[1] 陈庆山[1] 杨庆凯[1] 李文滨[1] 王文辉[1] 刘春燕[1] 陈立君[1] 刘海燕[1] 单继勋[1] 

机构地区：[1]东北农业大学,哈尔滨市150030

出　　处：《大豆科学》2004年第3期169-173,共5页Soybean Science

基　　金：国家"8 63"项目资助

摘　　要：针对中国大豆灰斑病 1号生理小种 ,以抗所有生理小种的品系东农 4 0 5 6 6为母本 ,以感 1号生理小种的品种东农 4 10为父本配制杂交组合 ,杂交得到F2 代后连续自交 3代得到F5代群体。该群体经人工接种灰斑病 1号生理小种后 ,利用BSA法对 5 0 0个SSR标记进行筛选 ,其中 3个标记Satt5 6 5、SOYGPATR和Satt396在抗、感池间表现出稳定的多态性 ,并且在F2 代个体中表现出抗性与多态性协同分离的趋势。这 3个标记与抗性基因的连锁顺序为Satt5 6 5 -SOYGPATR-Hrcs1-Satt396 ,它们与抗性基因的连锁距离分别为 12 .7cM、6 .5cM、14 .7cM。推测抗大豆灰斑病 1号生理小种的基因可能位于C1连锁群上。In aim to find the SSR markers linked to resistance gene against frogeye leaf spot in Chinese soybean, bulked segregant analysis was applied to F 5 populations derived from cross between the resistant line Dongnong40566 and the susceptible line Dongnong410, self-crossing for three generations after F 2.Three SSR markers including Satt565,SOYGPATR and Satt396 were identified from 500 SSR markers, the linkage order is Satt565-SOYGPATR-Hrcs1-Satt396, and the distance to the resistance locus is 12.7, 6.5 ,14.7cM respectively. These three markers and the resistance locus were maped to soybean linkage group C1.

关 键 词：大豆 灰斑病 抗性基因 SSR标记 品系 杂交组合 

分 类 号：S565.1[农业科学—作物学]