不同浓度糖氮对离体穗培养小麦粒重和叶片膜脂过氧化作用的影响  

Effects of Different Concentrations of Sucrose and Glutamine in Culture Solu-tion on Grain Weight and Cell Membrane Lipid Peroxidation in Flag Leaf ofWheat After the Ear Culture in vitro

作　　者：周琴[1] 姜东[1] 戴廷波[1] 荆奇[1] 曹卫星[1] 

机构地区：[1]南京农业大学农业部作物生长调控重点实验室,南京210095

出　　处：《植物生理学通讯》2004年第4期443-446,共4页Plant Physiology Communications

基　　金：国家自然科学基金 (30170544;30200166);教育部博士点基金 (2000030707);江苏省自然科学基金(BK2002205;BK2001063)。

摘　　要：在相同浓度配比的蔗糖和谷氨酰胺培养液中培养小麦品种扬麦9号和徐州26号的离体穗,检测不同水平的糖氮对小麦粒重及叶片膜脂过氧化作用的影响。结果表明,在一定浓度范围内,提高培养液中蔗糖和谷氨酰胺供应水平后,单粒重、叶片的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性提高,膜脂过氧化产物丙二醛(MDA)含量降低;培养液中糖氮水平过高,叶片的SOD和CAT活性则下降,MDA含量升高,籽粒单粒重下降。When the concentrations of sucrose and glutamine in the culture medium were lower than 60 and 6gL-1, respectively, the single grain weight and the activities of superoxide dismutase (SOD) and catalase (CAT)increased, and the content of malondialdehyde (MDA) in flag leaves decreased. However, excessive supply ofsucrose and glutamine reduced the activities of SOD and CAT, and increased MDA content in flag leaves, whilethe final single grain weight decreased. The results indicated that excessive levels of sucrose and glutamine inthe liquid medium were unfavorable for ear growth, and thus reduced grain weight in wheat.

关 键 词：蔗糖 谷氨酰胺 离体穗 粒重 小麦 叶片 超氧化物歧化酶 过氧化氢酶 

分 类 号：S512.1[农业科学—作物学]