盐胁迫下转甜菜碱醛脱氢酶基因水稻幼苗耐盐生理的研究  

Study on the Physiology of Transgenic Rice Seedlings with Gene of Betaine Aldehyde Dehydrogenase Gene under Salt Stress

作　　者：孙耀中[1] 东方阳[1] 陈受宜[2] 杨晓玲[1] 刘永军[1] 郭学民[1] 

机构地区：[1]河北科技师范学院生命科学系,河北昌黎066600 [2]中国科学院遗传研究所,北京100000

出　　处：《华北农学报》2004年第3期38-42,共5页Acta Agriculturae Boreali-Sinica

基　　金：河北省秦皇岛市科学技术局重点资助课题(2002-04)

摘　　要：在NaCl浓度0,3.0,5.0,7.0 g／L下,对转甜菜碱醛脱氢酶(BADH)基因水稻的两个品系51-22,52-7及其受体亲本中花8号进行幼苗耐盐性试验。结果表明:转.BADH基因水稻比其受体亲本耐盐性强,在高NaCl浓度(5.0,7.0 g／L)胁迫下,甜菜碱醛脱氢酶基因可以提高盐胁迫下水稻幼苗过氧化氢酶(CAT)活性,提高根系活力和叶绿素含量,稳定细胞膜的透性,降低植株体内Na+的积累,从而减轻幼苗受盐害的程度。盐胁迫下水稻幼苗的CAT活性、SNa+／K+、叶Na+／K+、叶绿素含量是影响幼苗生长的主要生理指标。Salt stress experiments of rice seedlings of two lines with a gene encoding for BADH,51 - 22,52 -7 and the recipient Zhonghua No. 8, were conducted under the NaCl concentration of 0,3.0,5.0,7.0 g/L. The results showed that the rice cultivars with a gene encoding for BADH demonstrated higher salt tolerance than its recipient; The gene encoding for BADH could reduce the salt injury by increasing CAT activity, root activity, chlorophyll content and cell membrane osmosis,and by decreasing the Na+ concentration in the seedlings under high NaCl stress (NaCl concentration:5.0,7.0 g/L) ;The CAT activity, SNa*/K+ and chlorophyll content are the main physiological indexes affecting the seedling growth.

关 键 词：盐胁迫 甜菜碱醛脱氢酶基因 转基因水稻 幼苗 耐盐生理 

分 类 号：S511[农业科学—作物学]