地塞米松磷酸钠脂质体的HPLC测定  

Determination of Dexamethasone Sodium Phosphate Liposomes by HPLC

作　　者：郭丹[1] 李国锋[1] 杨西晓[1] 侯连兵[1] 

机构地区：[1]第一军医大学南方医院药学部,广东广州510515

出　　处：《中国医药工业杂志》2004年第6期356-357,共2页Chinese Journal of Pharmaceuticals

基　　金：广东省自然科学基金(020056);广东省医学科研基金(A2002371)

摘　　要：建立了反相高效液相色谱法检测地塞米松磷酸钠脂质体的含量。采用Nucleosil 100-5 C18色谱柱,流动相为甲醇-0.34%磷酸二氢钾溶液(60∶40),检测波长240nm。地塞米松磷酸钠在0.78～50mg/ml浓度范围内线性关系良好(r=0.9997),方法平均回收率为99.6%,RSD为2.1%。A RP-HPLC method for determination of dexamethasone sodium phosphate liposomes was established.A Nucleosil 100-5 C18 column was used with the mobile phase of methanol-0.34% potassium dihydrogen phosphatesolution (60∶40), at the detection wavelength of 240nm. The calibration curve was linear in the concentration range of0.78～50mg/ml(r=0.9997). The average recovery was 99.6% with RSD of 2.1%.

关 键 词：地塞米松磷酸钠 脂质体 反相高效液相色谱 测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]