单引物PCR扩增DNA指纹图谱的稳定性研究  

STUDIES ON CONSTANCY OF DNA FINGERPRINTS AMPLIFIED BY SINGLE-PRIMED PCR

作　　者：张宏[1] 杨树青[1] 毛裕民[1] 王先敏[1] 徐迅[1] 劳晔 丁豪波 温俊娥 

机构地区：[1]复旦大学遗传学研究所

出　　处：《复旦学报（自然科学版）》1993年第4期399-405,共7页Journal of Fudan University：Natural Science

摘　　要：以人胶原蛋白基因下游一段DNA的互补序列设计引物,对人染色体DNA进行单引物PCR扩增,在低温退火的条件下,这种引物与DNA模板的一处完全配对,并且可以随机地结合在其他一些位置上.由此进行单引物PCR扩增,它们的扩增产物形成了稳定的引物——模板特异的DNA指纹图谱.本文所建立的单引物PCR扩增技术在类似的研究中均可获得稳定的实验结果,具有一定的应用价值.Human chromosome DNA was amplified by single-primed PCR with the primer designed from the complementary sequence of downstream site inthe human collagen gene. In low annealing temperature,this primer can match not only completely to a definite site in the template DNA, but also randomly to other sites. The products amplified by single-primed PCR formed DNA fingerprints with the specificity of primer-template. The method described here also works well in analogous studies.

关 键 词：引物 PCR扩谱 DNA 指纹图谱 

分 类 号：Q523[生物学—生物化学]