栖热菌(Thermus)的DNA转化研究  

STUDIES ON THE TRANSFORMATION IN Thermus BY CHROMOSOME DNA

作　　者：杨树青[1] 张宏[1] 毛裕民[1] 王先敏[1] 

机构地区：[1]复旦大学遗传学研究所

出　　处：《复旦学报（自然科学版）》1993年第4期460-464,共5页Journal of Fudan University：Natural Science

摘　　要：由栖热菌FDB8(Thermus sp.FDB8)提取的染色体DNA对栖热菌FD3009(Thermus sp.FD3009)进行了转化.用丝裂霉素C选择培养基对转化子进行选择,共获得3个转化子.它们的菌落色泽、对丝裂霉素C的抗性强度和耐热DNA聚合酶活性均介于供体菌和受体菌之间,而其菌体生长速度和破壁难易程度则优于供体菌和受体菌.结果表明,转化子是由供体菌FDB8的染色体DNA转化了受体菌FD3009所致,同时还可由转化子进一步选育出抗丝裂霉素C的耐热DNA聚合酶高产菌株.Thermus sp. FD3009 was transformed by Thermus sp. FDB8 chromosome DNA. Three transformants were obtained using the selecting medium of mitomycin C. These transformants show intermediate characteristics between the FD3009 and FDB8 with respect to their cell shape, clone colour, mitomycin C resistance and thermostable DNA polymerase activity.These indicate that the transformants ar(?) resulted from transforming FD3009 by FDB8 chromosome DNA.

关 键 词：栖热菌 转化 DNA 染色体 育种 

分 类 号：Q939.03[生物学—微生物学]