加压溶剂提取-高效薄层扫描法测定三七中皂苷类成分  

Analysis of Saponins from Panax notoginseng Using Pressurized Solvent Extraction Coupled with High Performance Thin Layer Chromatography

作　　者：万建波[1] 李绍平[1] 简家荣[1] 孔令义[2] 王一涛[1] 

机构地区：[1]澳门大学中华医药研究所 [2]中国药科大学天然药物化学研究室,南京210038

出　　处：《中国天然药物》2004年第4期215-218,共4页

摘　　要：目的 :建立加压溶剂提取 高效薄层扫描法测定三七中皂苷类成分含量的方法。方法 :采用加压溶剂提取三七中皂苷类成分 ,高效薄层扫描进行含量测定 ,使用高效薄层板、半自动点样、自动展开 ,10 %硫酸乙醇液显色 ,光密度扫描 ,测定波长 5 34nm ,参比波长 70 0nm。结果 :人参皂苷Rb1、Rd、Rg1和三七皂苷R1的线性范围为 :0 4 0 2～ 2 0 10 μg(r=0 9995 ) ;0 15 4～ 1 2 75 μg(r =0 996 5 ) ;0 198～ 1 980 μg(r =0 9998)和 0 15 6～1 4 0 0 μg(r=0 9978) ,回收率在 95 3～ 99 3%之间。 结论 :高效薄层扫描法可同时测定三七中的人参皂苷Rb1、Rd。AIM:To develop a method for analysis of saponins from panax notoginseng using pressurized solvent extraction coupled with high performance thin layer chromatography. METHOD:pressurized solvent extraction and HPTLC system equipped with applicator,auto-development,densitometer were used. The detection wavelength was 534nm(reference wavelength:700nm). RESULT:The standard curves of ginsenosides R_(b1),R_d,R_(g1) and notoginsenoside R_1 showed good linearity in the range of 0.402～2.010 μg(r=0.9995),0.154～1.275 μg(r=0.9965),0.198～1.980 μg(r=0.9998)and 0.156～1.400 μg(r=0.9978) respectively. The recoveries were between 95.3% and 99.3%. CONCLUSION:The developed method could be used to determine the content of saponins in panax notoginseng.

关 键 词：高效薄层扫描法 加压溶剂提取 三七皂苷 人参皂苷 含量测定 样品制备 精密度 

分 类 号：R284.1[医药卫生—中药学]