流式细胞术在成分血残留白细胞检测中的应用  

A flow cytometric method for the determination of residual leukocytes in blood components

作　　者：李丽[1] 王明元[1] 程根林 徐惠新[1] 周群刚[1] 夏毓伟 曹维娟[1] 张辉[1] 

机构地区：[1]江苏省苏州市红十字中心血站,215006

出　　处：《临床输血与检验》2004年第3期177-179,共3页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的　探讨流式细胞术 ( FCM)绝对计数法在成分血残留白细胞检测中的应用价值。方法　采用 FCM绝对计数法检测不同稀释度的浓缩血小板样本中白细胞残留量 ,并与 Nageotte血细胞计数板法比较。结果　两种方法计数微量白细胞具有高度相关性 ( r=0 .9964,P<0 .0 0 1 )。低浓度白细胞计数时 ,FCM法变异系数小于 Nageotte计数板法。两种方法检测结果相比 ,Nageotte计数板法计数值偏低。结论　流式细胞术绝对计数法具有快速、客观、精确和重复性好等优点 ,是一种稳定的检测成分血中微量残存白细胞数的方法。Objective To study the application of flow cytometric absolute counting method for counting residual leukocytes in blood components. Methods Residual leukocytes in platelet concentrates was counted by flow cytometric absolute counting method using fluorescent microbeads and Nageotte hemocytometry. Results Two kinds of methods correlated significantly (r=0.996 4, P<0.001). In low leukocyte counting, coefficient of variation of flow cytometric method was lower than that of Nageotte chamber. Conclusion The method of FCM provides a rapid, reliable, objective, and easily reproducible tool for measurement of residual leakocytes in blood components.

关 键 词：残留白细胞 流式细胞术 Nageotte血细胞计数板法 

分 类 号：R331.1[医药卫生—人体生理学] R392.11[医药卫生—基础医学]