黄精多糖对脂肪组织分泌的生物活性物质致肝细胞HepG_2分泌CRP的影响  

The effect of polygona-polysaccharose on CRP release stimulated by HepG_2

作　　者：吴燊荣[1] 李友元[2] 王小清[2] 王秀华[2] 唐汉权[2] 黄志凌[2] 肖洒 

机构地区：[1]中南大学湘雅医学院附属海口医院心内科,海口570208 [2]中南大学湘雅二院老年病科,长沙410011 [3]海南省海口市中医药学校,海口570208

出　　处：《中国新药杂志》2004年第6期502-504,共3页Chinese Journal of New Drugs

基　　金：海南省自然科学基金资助重点项目(No.30223)

摘　　要：目的:观察黄精多糖对脂肪组织分泌的生物活性物质致肝细胞HepG2分泌VRP的影响。方法:取脂肪组织培养液刺激肝细胞HepG2培养液,观察基础状态及黄精多糖对C-反应蛋白(CRP)分泌的影响。结果:内脏及皮下脂肪培养液均可刺激肝细胞HepG2产生CRP,且腹腔内脂肪培养液刺激HepG2合成CRP[(3.84±0.92)ng·mL-1]强于皮下脂肪[(2.45±0.75)ng·mL-1,P<0.01]。黄精多糖可抑制肝细胞HepG2分泌CRP,对皮下及腹腔脂肪培养液所产生的刺激作用分别抑制达45.8%和66.7%。结论:脂肪组织分泌的生物活性物质可刺激肝细胞HepG2分泌CRP,提示脂肪组织可通过CRP致动脉粥样硬化形成。黄精多糖可抑制CRP分泌,具有抗动脉粥样硬化作用。Objective:To investigate the effect of biological substances from adipose tissue on HepG2 and the mechanism of anti-atherosclerosis by polygona-polysaccharose. Methods: HepG2 cells were cultured in adipose tissue culture media to investigate the status of C-reactive protein (CRP) release under basic condition and co-existence with polygona-polysaccharose. Results:The culture media of both omental and subcutaneous adipose tissue stimulated the HepG2 to produce CRP. The level of CRP stimulated by omental adipose tissue culture media was higher than that by subcutaneous adipose culture media,i.e.,[(3.84±0. 92)ng·mL-1 vs (2.45±0.75) ng·mL-1, P< 0. O1]. Polygona-polysaccharose suppressed the HepG2 to release CRP, whose inhibition rate in omental and subcutaneous adipose culture media was 66. 7% and 45. 8% , respectively. Conclusion: The biological substances produced from adipose tissue could stimulate the HepG2 cells to release CRP, which suggests that the adipose tissue may cause atheroslerosis by impact of CRP.Since polygona-polysaccharose may inhibit the CRP release,it demonstrates an effect of anti-atherosclerosis.

关 键 词：C—反应蛋白 黄精多糖 组织培养 细胞培养 

分 类 号：R972.5[医药卫生—药品] R541.4[医药卫生—药学]