HPLC法测定家兔血浆中葛根黄豆苷元衍生物DZ18的含量  

Determination of Daidzei Derivative DZ18 in Rabbits' plasma by HPLC

作　　者：姜铁夫 [1] 陈济民 [2] 佟志清 [1] 李媛 [1] 曲辉 [1] 

机构地区：[1]中国人民解放军第463医院,药剂科,辽宁,沈阳,110042 [2]沈阳药科大学,辽宁,沈阳,110016

出　　处：《解放军药学学报》2004年第3期219-220,共2页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家新药研究基金资助课题;No .9690 10 5 55

摘　　要：目的　建立测定家兔血浆中葛根黄豆苷元衍生物DZ18含量的高效液相色谱法。方法　采用HPLC法 ,使用ODS -C18色谱柱 ,流动相 :甲醇 -水 -磷酸 (5 0∶5 0∶0 .1) ,检测波长 2 6 0nm。结果　DZ18浓度在 0～ 10 0 μg·ml-1范围内 ,线性关系良好 ,r=0 .9998。回收率在 89%以上 ;日内、日间精密度均低于 5 %。Aim To establish an HPLC method for the determination of daidzei derivative DZ18 in rabbits' plasma.Methods The reserved C 18 column was used.The mobile phase consisted of methanol-water-phosphoric acid(50∶50∶0.1).Detective wave was 260nm.Results The linear range was from 0 to 100μg·ml -1,r=0.999 8.The average recovery was above 89%.Conclusion The method can be used in the determination of daidzei derivative DZ18 in rabbits' plasma.

关 键 词：HPLC法 含量测定 家兔 血浆 葛根黄豆苷元衍生物 DZ18 药动学 

分 类 号：R969.1[医药卫生—药理学]