基因治疗新策略:转录因子诱捕方法的应用进展  

New Strategies for Gene Therapy-transcription Factor "Decoy" Method by Using Double-stranded Decoy Oligonucleotide

作　　者：席锐[1] 陆国平[1] 

机构地区：[1]上海第二医科大学附属瑞金医院心内科上海市，200025

出　　处：《医学分子生物学杂志》2004年第3期172-174,共3页Journal of Medical Molecular Biology

基　　金：上海市科委基础研究重大项目(No.02JC14037)

摘　　要：转录因子诱捕方法是一种在转录前和转录水平调控靶基因表达的技术,该技术利用双链诱捕性寡核苷酸(double-stranded decoy oligonuezleotide)竞争性结合转录因子的特异序列识别结构域,抑制转录因子与启动子序列的特异性结合,从而抑制下游基因的转录起始。近几年在基因治疗方面取得了明显进展。Transcription factor 'decoy' strategy is a technique regulating target gene expression at pretranscriptional and transcriptional levels. This method takes advantages of double-stranded decoy oligonucleotide competing with authentic cis element for binding to the specific sequences recognizing domain of transcription factor, thereby inhibiting the initiation of transcription of down-stream gene. In recent years, great progress has been made in gene therapy. This article reviews the recent achievements of gene therapy by using the technique.

关 键 词：基因治疗 转录因子 诱捕性寡核苷酸 

分 类 号：R454.9[医药卫生—治疗学]