随机扩增多态性DNA分子标记研究进展及在中药鉴定中的应用  

Advances in Molecular Marking of Random Amplified Polymorphic DNA(RAPD) and Its Applications in the Authentication of Traditional Chinese Drug(TCD)

作　　者：刘石泉[1] 周根余[1] 李小军[1] 路群[1] 余庆波[1] 

机构地区：[1]上海师范大学生命与环境科学学院生物系遗传学实验室,200234

出　　处：《时珍国医国药》2004年第7期431-434,共4页Lishizhen Medicine and Materia Medica Research

基　　金：上海市高等学校科学技术发展基金项目 (No.0 3DZ0 2 )

摘　　要：随机扩增多态性 DNA是一种兼稳定性和多态检出率高的 DNA分子标记技术之一 ,本文综述了 RAPD技术原理、操作过程、优势、不足和应对措施以及该技术的扩展、在中药分类、鉴定等中的应用。Random amplified polymorphic DNA (RAPD), a DNA molecular marking technology, is one of reliable and powerful approaches in detecting polymorphism loci. This paper presents the RAPD's principle, process, superiorities, shortcoming and its remedied measures. Expansibility of RAPD and its applications in the authentication of traditional Chinese drug (TCD) are also studied.

关 键 词：随机扩增多态性DNA RAPD扩展 中药鉴定 应用 

分 类 号：R282.5[医药卫生—中药学]