类风湿性关节炎患者血清和滑液中血管内皮生长因子与病损程度的相关性  

Relation of vascular endothelial growth factors in serum and synovial fluid with lesion degree in patients with rheumatoid arthritis

作　　者：刘巧红[1] 沈凌汛[1] 吴清敏 滕云[2] 王慧[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院风湿科,湖北省武汉市430022 [2]华中科技大学同济医学院医学生物学系,湖北省武汉市430030

出　　处：《中国临床康复》2004年第21期4242-4243,共2页Chinese Journal of Clinical Rehabilitation

基　　金：湖北省科学技术委员会科研资助项目(2002AA301C47)~~

摘　　要：目的:研究类风湿关节炎(rheumatoidarthritis,RA)患者血清、滑液中血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)蛋白水平,探讨其在RA致病中的作用。方法:应用双抗夹心酶联免疫吸附(ELISA)法分别测定RA患者(n=36)血清、滑液中VEGF蛋白水平。以骨关节炎(n=9)及因外伤截肢的正常人(n=13)作对照。结果:RA患者血清、滑液中VEGF水平(ng/L)分别为186±77,262±84;而对照组分别为48±17,63±27。RA患者血清、滑液中VEGF蛋白水平均显著高于对照组(t=1.63,P=0.008;t=1.72,P=0.006),且与关节疼痛数、关节肿胀数、晨僵时间、红细胞沉降率、C反应蛋白、类风湿因子滴度均呈正相关(r=0.73～0.83,P均<0.05)。结论:RA患者VEGF蛋白的过度表达是导致RA发病的重要因素。AIM:To study the expression level of vascular endothelial growth factor(VEGF) in serum and synovial fluid of patients with rheumatoid arthritis(RA) and to explore its pathological role in RA. METHODS:The VEGF protein levels in serum and synovial fluid,respectively,were determined by enzyme linked immunosorbent assay(ELISA) in patients with RA(n=36),and 9 patients with osteoarthritis and 13 amputated healthy persons because of trauma were taken as the control group. RESULTS:The mean VEGF levels(ng/L) in serum and synovial fluid were 186±77 and 262±84 in RA patients, significantly higher than those in the control group(48±17 and 63±27)(t=1.63,P=0.008;t=1.72,P=0.006).The levels of VEGF protein in both serum and synovial fluid of patients with RA were significantly associated with the number of joint pain,swollen joint,duration of morning stiffness,erythrocyte sedimentation rate(ESR),C reactive protein(CRP) and rheumatoid factor level respectively (r=0.73-0.83,P< 0.05).CONCLUSION:The overexpression of VEGF is an important factor for the pathogenesis of RA.

关 键 词：类风湿性关节炎 血清 滑液 血管内皮生长因子 病损程度 双抗夹心酶联免疫吸附 测定 

分 类 号：R593.22[医药卫生—内科学]