复方连竹胶囊对糖尿病大鼠脂肪细胞肿瘤坏死因子-α表达的影响  

Influence of fufang lianzhu capsule on the expression of lipoblast tumor necrosis factor alpha in rats with diabetes

作　　者：金光香[1] 段文卓[1] 徐丽华[1] 李锋杰[1] 

机构地区：[1]潍坊医学院免疫及病原生物学教研室,山东省潍坊市261042

出　　处：《中国临床康复》2004年第21期4255-4257,共3页Chinese Journal of Clinical Rehabilitation

基　　金：山东省中医药管理局科研基金项目(96026)~~

摘　　要：目的:研究中药复方连竹胶囊对糖尿病大鼠脂肪细胞(lipoblast)肿瘤坏死因子-α(tumornecrosisfactor-α,TNF-α)mRNA表达的影响,寻找防治糖尿病及微血管炎性并发症的有效途径。方法:复制糖尿病大鼠模型,采用核酸原位杂交、组织培养及生化技术分析测定各项指标。结果:糖尿病组大鼠脂肪细胞TNF-αmRNA的表达明显增强,血糖、血脂、糖化血红蛋白、尿蛋白、醛糖还原酶活性及丙二醛含量均明显高于正常对照组(P<0.05);糖尿病组大鼠血浆NO含量明显低于正常对照组(P<0.05)。中药治疗组以上各指标均较糖尿病组有明显改善,但与正常对照组比较差异无显著性意义(P>0.05)。形态学观察,糖尿病组大鼠肾血管及视网膜血管均有炎性浸润性增生改变;中药治疗组其病理改变较糖尿病组减轻。结论:复方连竹胶囊对糖尿病大鼠有明显的治疗作用,并能降低脂肪细胞TNF-αmRNA的表达,减低微血管炎性并发症的产生。AIM:To investigate the effect of fufang lianzhu capsule on the mRNA expression of lipoblast tumor necrosis factor alpha(TNF α) in rats with diabetes,so as to probe into the prevention so as to cure method against diabetes and complications of microvascular inflammation. METHODS:The model of diabetic rats was set up and various indexes were determined with ribonucleic acid hybridization in situ,tissue culture and biochemical technique method. RESULTS:The mRNA expression of lipblast TNF αof rats with diabetes strengthened significantly,and blood glucose,blood lipid, glycosylation ferrohemoglobin,protein in urine,aldose reductase activity and propylene aldehyde content were all remarkably higher than those of the normal control group(P< 0.05);and the plasm NO content was lower than that of the normal control group(P< 0.05).The indexes mentioned above in the TCM group were improved as compared with those of the diabetes group,but without significant difference(P >0.05).Morphologically observed,flammatory infiltration proliferation changes happened in blood vessel of kidney and retina.Pathologic changes in diabetes group were lighter than those in the TCM group.CONCLUSION:Fufang lianzhu capsule has obvious therapeutic effect on diabetic rat,and can decrease the lipoblast TNFαmRNA expression and flammatory complications of microvessel.

关 键 词：复方连竹胶囊 糖尿病 大鼠 脂肪细胞 肿瘤坏死因子-Α 微血管炎性并发症 

分 类 号：R259.872[医药卫生—中西医结合] R259.43[医药卫生—中医内科学]