诱导性一氧化氮合成酶在大鼠肝移植小移植物模型早期损伤中的表达及意义  

Expression and role of iNOS in early injury of small graft in rat liver transplantation

作　　者：吴晓峰[1] 钱建民[1] 王学浩[1] 

机构地区：[1]南京医科大学第一附属医院肝脏外科,210029

出　　处：《中华实验外科杂志》2004年第7期821-823,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨诱导性一氧化氮合成酶(iNOS)在大鼠肝移植小移植物早期损伤中的表达及意义。方法选取雄性SD大鼠,随机分成3组假手术组(正常对照组,A组),缺血再灌注组(B组),小移植物组(C组)。比较各组的肝功能指标的变化、肝脏脂质过氧化产物丙二醛(MDA)和超氧化物歧化酶(SOD)的浓度、肝脏组织病理组织学和透射电镜改变、逆转录聚合酶链反应(RTPCR)检测iNOS在肝组织中的表达。结果A组肝功能指标显著低于其余两组(P<0.05);肝功能指标峰值均出现在术后6h,随后逐渐下降;肝功能指标峰值依次为C组>B组>A组(P<0.05)。A组MDA显著低于其余两组,肝脏MDA浓度C组>B组>A组(P<0.05);SOD呈相反变化。光镜检查、电镜检查B、C两组均可见到肝脏再灌注损伤后典型的病理变化。再灌注后B、C组肝组织中iNOS表达水平显著升高,在6h时达到峰值,随后逐渐下降;至12和24h仍维持远高于对照组的水平。C组iNOS峰值组显著高于B组。结论iNOS在小移植物损伤中积极地参与了缺血再灌注损伤过程。Objective To investigate expression and role of iNOS in early injury of small graft in rat liver transplantation.Methods Male Sprague Dawley rats were divided randomly into three groups: sham group (control,group A,8 rats),ischemia reperfusion group (group B,30 rats),small graft group (group C,30 rats).Changes of TB、ALT and AST in serum,MDA and SOD in liver,pathology of liver and expression of iNOS in liver of three groups were compared.Results TB、ALT and AST of group A were lower significantly than these of group B and group C ( P <0.05).The peak of TB、ALT and AST was observed at 6h,then descended.The peak of TB、ALT and AST of group C were highest and these of group A were lowest.MDA of group A was lower than other groups,MDA of group C was higher than group B( P <0.05),the change of SOD was contrary to MDA.Pathology in light and electronic of group B and C were typical changes after ischemia reperfusion injury.Expression of iNOS after reperfusion of group B and C increased significantly and reached peak at 6h,then descended.The level of iNOS in 12 24 h was higher than control.The peak of iNOS of group C was significantly higher than group B.Conclusion These results indicated that iNOS was involved actively in early injury of small graft in rat liver transplantation.

关 键 词：诱导性一氧化氮合成酶 大鼠 肝移植 移植物模型 损伤 表达 

分 类 号：R657.3[医药卫生—外科学]