HPLC测定益心酮滴丸中的黄酮苷  

Determination of flavoniod-glycosides in Yixintong pills by HPLC

作　　者：苏娟[1] 张卫东[1] 周耘[1] 周静[2] 顾正兵[1] 

机构地区：[1]第二军医大学药学院,上海200433 [2]第二军医大学长征医院药学部,上海200037

出　　处：《中国中药杂志》2004年第6期525-527,共3页China Journal of Chinese Materia Medica

摘　　要：目的 :建立益心酮滴丸的含量测定方法。方法 :色谱柱AgilentZORBAXExtend -C18(4.6mm× 2 5 0mm ,5 μm) ,流动相四氢呋喃 甲醇 乙腈 水 醋酸 (30∶3∶4∶12 0∶3) ,检测波长 330nm。 结果 :牡荆素鼠李糖苷 (SZ 1)在0 .0 4 4～ 1.78μg(r=0 .9999) ,牡荆素葡萄糖苷 (SZ 2 )在 0 .0 4 2～ 0 .85 μg(r=0 .9999)线性关系良好 ,加样回收率分别为 10 0 .0 %和 99.2 % ,RSD分别为 0 .92 %和 1.8%。结论 :本方法可有效地控制益心酮滴丸的质量。Objective: To develop an HPLC method for determining flavoniod-glycosides in Yixintong pills. Method: Agilent ZORBAX Extend-C 18 (4.6 mm×250 mm,5 μm) was used with tetrahydrofuran-methanol-acetonitrile-water-acetic acid (30∶3∶4∶120∶3) as mobile phase. Detection wavelength was 330 nm. Result: Good linearities of rhamnosylvitexin and vetexin-glucoside were obtained within the range of 0.044～1.78 μg (r=0.999 9) and 0.042～0.85 μg (r=0.999 9);the average recoveries were 100.0% and 99.2%;RSD were 0.92% and 1.8%,respectively. Conclusion: This method can be used for quality control of Yixintong pills.

关 键 词：HPLC 益心酮滴丸 黄酮苷 牡荆素葡萄糖苷 高效液相色谱法 

分 类 号：R286[医药卫生—中药学]