骨碎补提取液对实验性牙槽骨吸收疗效的研究  

Therapeutic effect of aqueous-extract from a traditional Chinese medical herb Drynaria fortunei on rat experimental model of alveolar bone resorption

作　　者：陈莉丽[1] 唐琪[1] 严杰[2] 

机构地区：[1]浙江大学医学院附属第二医院口腔科,浙江杭州310009 [2]浙江大学医学院病原生物学教研室,浙江杭州310006

出　　处：《中国中药杂志》2004年第6期549-553,共5页China Journal of Chinese Materia Medica

基　　金：浙江省科技厅资助项目 (99110 3 115 )

摘　　要：目的 :建立实验性大鼠牙槽骨吸收模型 ,了解中药骨碎补 (DFS)对大鼠牙槽骨吸收的疗效。方法 :采用SD大鼠局部注射大肠杆菌内毒素 (E LPS)建立牙槽骨吸收模型。制备DFS水相提取液。建模大鼠用DFS水提液每天灌胃 1次 ,分别用药 10 ,2 0 ,30d。采用血清碱性磷酸酶 (ALP)活性、钙离子 (Ca2 + )和骨钙素 (OC)浓度测定 ,以及牙体 牙周联合标本骨密度 (BMD)、抗酒石酸酸性磷酸酶 (TRAP)染色破骨细胞计数、HE染色组织病理学检查 ,评价DFS水提液治疗实验性牙槽骨吸收的疗效。结果 :与相应阴性对照组比较 ,用药 10d大鼠的实验牙位骨密度显著升高 (P <0 .0 5 ) ,牙周破骨细胞消失 (P <0 .0 5 ) ,Howship陷窝明显减少 ,大多出现根分叉区有成骨细胞附着的新生未钙化骨样基质 ,且第 2 0 ,30天检查结果与第 10天相似。用药 10～ 30d大鼠的血清标本中ALP活性、Ca2 + 和OC浓度与对照组比较均无显著性差异 (P >0 .0 5 )。结论 :DFS水提液对大鼠实验性牙槽骨吸收有明确的疗效 ,能抑制骨质吸收、促进骨质再生。血清ALP活性、Ca2 + 和OC浓度不能作为观察动物模型中牙槽骨吸收及再生的有效指标。Objective: To establish a rat model of experimental alveolar bone resorption and understand the therapeutic effect of a traditional Chinese medical herb Drynaria fortunei (DFS) on the rats suffering from alveolar bone resorption. Method: A SD rat model with experimental alveolar bone resorption was established by using injection with E.coli Lipopolysaccharide (E-LPS) in local tissue of the animals. A DFS preparation (DFS aqueous-extract) was extracted with distilled water. The modeled rats were administrated by perfusion with DFS aqueous-extract at a dosage of 15 g crude drug/kg once a day for 10,20 and 30 d,respectively. The effects of DFS aqueous extract on experimental alveolar bone resorption were estimated by detections of serum alkali phosphate (ALP) activity,Ca 2+ and osteocalcin (OC) levels,and TRAP stained osteoclast count,bone mineral density (BMD) measurement and HE stained histopathological examination of the tooth-periodontal samples. Result: In comparison with the controls,BMD values of the alveolar bones at experimental tooth sites of the tested rats with DFS aqueous-extract for 10 d administration were obviously increased (P<0.05). In the tooth-periodontal samples from the rats for 10 d administration,disappearance of the osteoclast,decrease of Howship's lacuna numbers and formation of new non-calcified bone-like matrix attached by osteoblasts in alveolar bone at the root furcation from most of the samples occurred. Similar examining results in the tooth-periodontal samples from the rats for 20 and 30 d administrations were obtained,respectively. However,no statistically significant differences of the serum ALP activity,Ca 2+ and OC levels among the tested rats for 10～30 d treatment and the controls (P>0.05) were found. Conclusion: The DFS aqueousextract has exact therapeutic effect on rat experimental alveolar bone resorption through suppressing bone resorption and promoting bone regeneration. Serum ALP activity,Ca 2+ and osteocalcin (OC) levels can not be used as the effective index f

关 键 词：骨碎补提取液 实验性 牙槽骨吸收 DFS 骨钙素 

分 类 号：R285.5[医药卫生—中药学]