无毒棉籽水提物中抗抑郁活性成分的筛选研究  

Screening of the antidepressant components in aqueous extract of detoxified cottonseeds

作　　者：李云峰[1] 刘艳芹[1] 杨明[1] 王恒林[2] 赵毅民[1] 罗质璞[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850 [2]解放军第309医院麻醉科,北京100091

出　　处：《军事医学科学院院刊》2004年第3期250-251,263,共3页Bulletin of the Academy of Military Medical Sciences

基　　金：北京市自然科学基金资助项目 ( 70 42 0 5 2 )

摘　　要：目的 :筛选无毒棉籽水提物有效部位 (CTN)中抗抑郁活性成分。方法 :提取大脑皮质突触膜 ,以CTN或其中 5个黄酮类单体成分 (分别为CTN 4 ,CTN 7,CTN 8,CTN 9和CTN 10 )与突触膜共孵 ,放射免疫法测定腺苷酸环化酶 (adenylatecyclase ,AC)活性。结果 :与阳性药丁螺环酮 (5 HT1A受体部分激动剂 )类似 ,CTN及CTN 4 ,7,8,9,10均可剂量依赖性激活突触膜AC ,其中CTN 8在 5个黄酮类单体中活性最强。结论 :黄酮类化合物槲皮素 3 O 芹菜糖基芦丁糖苷 (即CTN 8)可能是CTN中最强的抗抑郁活性成分之一 ,并且可能是 5 HT1A受体激动剂。Objective: To screen the antidepressant components in aqueous extract of detoxified cottonseeds (CTN). Methods: The synaptic membrane of rat cerebral cortex was extracted and incubated with CTN or its five flavonoid compounds (CTN-4, 7, 8, 9, 10) respectively. The adenylyl cyclase (AC) activity was measured by radioimmunoassay. Results: Just like the positive drug buspirone, a 5-HT 1A receptor partial agonist, CTN and its five flavonoid compounds all activated AC in synaptic membrane in a dose-dependent manner, with compound CTN-8 being the most effective. Conclusion: The CTN-8, quercetin-3-O-apionyl- rutinoside, may be one of the most effective antidepressant components in CTN, and be a 5-HT 1A receptor agonist.

关 键 词：棉籽 腺苷酸环化酶 抗抑郁剂 药物筛选 

分 类 号：R965.1[医药卫生—药理学]