异种聚合血红蛋白抗原性检测方法的探讨  

Methods for examination of antigenicity of heterogeneous polymerized hemoglobin

作　　者：陈琳[1] 张亚东[1] 卜凤荣[1] 章金刚[1] 

机构地区：[1]军事医学科学院野战输血研究所,北京100850

出　　处：《军事医学科学院院刊》2004年第3期278-279,282,共3页Bulletin of the Academy of Military Medical Sciences

摘　　要：目的 :选择并建立异种聚合血红蛋白抗原性检测方法 ,为评价异种聚合血红蛋白对人有无抗原性提供参考。方法 :从速发型超敏反应、体液免疫反应、细胞免疫反应和抗原交叉反应检测聚合牛血红蛋白对异种动物的抗原性。结果 :家兔及豚鼠未发生速发型超敏反应。多次免疫后 ,家兔血清中总IgG无明显变化 ,但特异性IgG水平显著升高。B淋巴细胞转化检测表明 ,B淋巴细胞增殖活性增强 ,但与对照组相比无显著差异 (P >0 .0 5 )。聚合牛血红蛋白、人血红蛋白、兔血红蛋白抗原交叉反应结果显示 ,聚合牛血红蛋白与人血红蛋白存在抗原交叉反应 ,提示聚合牛血红蛋白与人血红蛋白在抗原结构上有同源性。结论 :该聚合牛血红蛋白与人血红蛋白在抗原结构上有同源性 ,除用其免疫的实验组动物血清特异性抗体水平显著升高外 ,其他检测指标无显著变化。根据免疫学理论 ,无论某异物刺激机体产生何种免疫应答反应 ,都应视为该异物存在抗原性 ,因此提示该聚合血红蛋白存在抗原性。Objective: To choose and establish the methods for examination of heterogeneous polymerized hemoglobin in order to offer the reference for evaluating the antigenicity of heterogeneous polymerized hemoglobin against human. Methods: Antigenicity of heterogeneous polymerized hemoglobin was examined for hypersensitivity, cell-mediated immunity reaction, humoral immunity reaction and cross-reaction of antigen. Results: The rabbit and guinea pig did not give rise to hypersensitivity. In immunized rabbits, the level of serum total IgG was normal, but the level of serum specific IgG was high. The examination of B lymphocytes showed that there was no significant difference (P>0.05) in comparison with control. Cross-reaction of antigen proved that bovine hemoglobin had cross-reaction with human hemoglobin. Suggesting that they may be homologous in the antigen structure. Conclusion: Although bovine hemoglobin and human hemoglobin are homologous, the level of the serum specific antibody is high in the immunized animal. According to the immunology theories, the polymerized hemoglobin has antigenicity.

关 键 词：异种聚合血红蛋白 抗原性 检测方法 血红蛋白测定 

分 类 号：R392.11[医药卫生—免疫学]