体外培养系统中各细胞组分在IL-18诱导的肿瘤特异性CTL中的作用  

Effects of Various Cell Components of Peripheral Blood to Induce Tumor-Specific CTL by IL-18 in the Cocultural System in vitro

作　　者：谭岩[1] 方艳秋[1] 段秀梅[1] 刘立华[1] 张雪松[2] 姜艳芳[1] 时阳[3] 刘桂英[3] 

机构地区：[1]吉林大学第一医院中心实验室,长春130021 [2]长春市中心血站,长春130051 [3]吉林大学第一医院肿瘤生物治疗中心,长春130021

出　　处：《现代免疫学》2004年第4期288-292,共5页Current Immunology

基　　金：卫生部临床学科重点科研资助项目(20013145);吉林省科技厅重点科研资助项目(20020403);吉林省卫生厅科研资助项目(020);长春市科委科研资助项目(0178S02);2002年吉林大学创新基金

摘　　要：应用rhIL 18在体外培养系统 (coculturesysteminvitro ,CCS )中诱导肿瘤特异性细胞毒性T淋巴细胞 (cytotoxicTlympho cyte ,CTL ) ,探索不同细胞在IL 18起动和促进肿瘤特异性免疫应答方面的作用。采用StemSepTM免疫磁性细胞分离法分离人外周血NK细胞、T细胞及树突细胞 (DC ) ,流式细胞仪分析细胞表型 ,12 5I UdR标记的细胞毒实验检测杀伤活性。结果表明 ,在肿瘤抗原存在的条件下 ,CCS中rhIL 18能够诱导并促进CTL介导的肿瘤特异性杀伤效应 ;并且 ,在rhIL 18诱导肿瘤特异性CTL的过程中 ,rhIL 18、NK细胞、DC及T细胞均起着十分重要的作用 ,缺少任一组份 ,均对诱导肿瘤特异性CTL有明显差异 (P <0 0 1)。提示在CCS中 ,rhIL 18诱导的NK细胞快速杀伤效应及DC的抗原提呈作用 ,在肿瘤特异性CTL产生过程中 ,均起着决定性的作用。The cocultural system in vitro (CCS) was used in the present study to induce tumor-specific CTL by IL-18 in order to investigate the effects of various cell components of peripheral blood to prime and enhance the tumor-specific immune responses. The immunoscreening with StemSep TM McAb-coupled magnetic beads was employed to isolate NK cells, T cells and dendritic cells (DC) from human peripheral blood, and the cell phenotype was determined by flow cytometry analysis. To examine the cytotoxic activity of the induced CTL the cytotoxicity test with labeled 125 I-UdR was used. It was found that the recombinant human IL-18 (rhIL-18) could induce and enhance the tumor-specific cytotoxic effects mediated by CTL in the presence of the tumor-specific antigens in the CCS. In addition, in this IL-18 induced tumor-specific cytotoxicity, rhIL-18, NK cells, T cells and DC, all exhibited very important effects on the cytotoxic effects, showing remarkable influences in the cytotoxicities in the absence of any one of these cells. These results suggest that both the rapid NK cytolysis induced by IL-18 and the antigen presenting activity of DC are critical to the induction of the tumor-specific activity of CTL in the cocultural system.

关 键 词：体外培养系统 rhIL-18 肿瘤杀伤效应 肿瘤特异性CTL 

分 类 号：R392.11[医药卫生—免疫学]