针刺对快速老化小鼠生长因子及受体基因表达的影响  

Effects of acupuncture on growth factors and expression of receptor genes in senescence accelerated mouse

作　　者：于涛[1] 于建春[1] 陆明霞[1] 王舒[1] 董承超[1] 丁晓蓉[1] 庞莹[1] 韩景献[1] 

机构地区：[1]天津中医学院第一附属医院老年病研究室,天津市300193

出　　处：《中国临床康复》2004年第22期4528-4529,共2页Chinese Journal of Clinical Rehabilitation

基　　金：天津市自然科学基金资助课题(013612911)~~

摘　　要：目的:探讨快速老化小鼠(senescenceacceleratedmouse,SAM)脑衰老的机制,揭示“益气调血、扶本培元”针法改善SAMP10脑衰老症状的部分机制。方法:选取7月龄雄性清洁级SAMP10小鼠20只,单纯随机分为2组,即SAMP10对照组、SAMP10“益气调血、扶本培元”针刺法组(简称针刺组);选取7月龄雄性SAMR110只,为SAMR1对照组。应用cDNAArray技术,观察快速老化小鼠SAMP10与正常老化小鼠SAMR1前脑588个基因表达的差异,同时观察“益气调血、扶本培元”针法对上述基因表达的影响。结果:SAMP10与SAMR1相比,胰岛素样生长因子ⅠA、胰岛素样生长因子Ⅱ前体、生长激素受体、雌激素受体伴快速老化,其表达分别下调了16,1.6,20.2和7.6倍,胰岛素样生长因子I受体α亚单位的表达则上调了1.6倍。针刺后其表达水平趋向于正常老化的R1品系,其表达分别上调11.5,4.1,17.1和3.8倍,上调1.8倍。结论:SAMP10脑衰老可能与胰岛素样生长因子系统、生长激素等的异常表达有关,针刺可改善上述生长因子的表达,进而起到延缓脑衰老作用。AIM:To explore the mechanism of brain senescence in senescence accelerated mou se(SAM) P10,and discover the partial mechanism of amelioration by “yiqi tiaoxue ,fuben peiyuan' acupuncture therapy on the brain senescent syndrome of SAMP10. METHODS:Twenty male SAMP10 of clean grade, aged 7 months old,were randomly div ided into 2 groups:SAMP10 control group and SAMP10 “yiqi tiaoxue,fuben peiyuan' acupuncture therapy group (acupuncture group). Another 10 SAMR1,aged 7 months o ld,were selected as the SAMR1 control group.The cDNA Array technique was used to observe the difference in the expression of 588 genes among the SAMP10 control group,SAMR1 control group and acupuncture group.Meanwhile,the effect of “yiqi t iaoxue,fuben peiyuan' acupuncture therapy on the expression of the above mention ed genes was detected. RESULTS:Compared with the SAMR1 control group, the expressions of insulin-lik e growth factor(ILGF) ⅠA,ILGF ⅡPROSOMA, growth hormone receptor and estrogen r eceptor combined with accelerated senescence were reduced by 16,1.6,20.2 and 7.6 times,while the ILGF I receptor alpha was up-ragulated by 1.6 times.After acup uncture therapy, the expressions showed a tendency to the normal senescence, the ir expressions were up-regulated by 11.5,4.1,17.1,3.8 and 1.8 times respectivel y. CONCLUSION:The abnormal expression of ILGF system and growth hormone receptor are possibly related with the senescence of SAMP10.The brain senescent syndrome of SAMP10 can be ameliorated by acupuncture through regulating the expression of genes.

关 键 词：针刺 小鼠 生长因子 生长因子受体 基因表达 衰老 

分 类 号：R339.38[医药卫生—人体生理学] R245[医药卫生—基础医学]