密骨颗粒对去卵巢大鼠TGF-β1表达的影响  

Effects of Mi Gu granule on expression of TGF-β1 in OVX osteoporotic rat

作　　者：任艳玲[1] 郑洪新[1] 杜松[1] 

机构地区：[1]辽宁中医学院基础部,沈阳110032

出　　处：《中国骨质疏松杂志》2004年第2期221-225,共5页Chinese Journal of Osteoporosis

基　　金：国家中医药管理局科研项目(2000-J-P-08);沈阳市科委科学基金(1022045-1-04)

摘　　要：目的 观察密骨颗粒对去卵巢大鼠TGF-β1表达的影响,探讨其对绝经后骨质疏松症的预防机制。方法 运用切除大鼠双侧卵巢方法建立骨质疏松症模型,应用中药密骨颗粒防治12周,采用双能X线骨密度测定法、Elisa、免疫组化SABC法、反转录聚合酶链方法,分别观察其对实验鼠离体骨密度、血清TGF-β1活性及其在骨组织中的蛋白和mRNA表达的影响。结果 中药密骨颗粒可以显著升高骨密度,提高血清TGF-β1活性,上调其在骨组织中的mRNA和蛋白表达水平,其蛋白广泛表达于胫骨干骺端。结论 中药密骨颗粒可通过调节TGF-β1活性及其mRNA表达水平而对去卵巢大鼠骨质疏松有预防或延缓发生的作用。Objective To study the effects of Chines herbal preparation Mi Gu granule on expression of TGF-β1 in OVX rats, and the mechanism of the Chinese medicine in preventing and treating menopausal osteoporo-sis . Methods Rat model of primary osteoporosis was developed by ovariectomy. After the rats were administered Mi Gu granule for 12 weeks, the bone mineral density (BMD) of the rat femur, the activity of the TGF-β1 in the rat serum, and the expression of TGF-β1 in the bone tissue were determined by DEXA, ELISA, immunohistoche-mistry, and RT-PCR, respectively. Results Mi Gu increased the BMD dramatically, stimulated the activity of TGF-β1 in the serum, premoted the expression of TGF-β1 mRNA in the bone tissue, and its protein expression was located mainly at the epiphysis of the bone. Conclusion Mi Gu prevents and delays the osteoporosis in OVX rats by regulating the activity of TGF-β1 and the expression level of the TGF-β1 mRNA.

关 键 词：密骨颗粒 去卵巢 大鼠 TGF-Β1 绝经后 预防 

分 类 号：R274.91[医药卫生—中西医结合]