幼鼠髋臼发育与机械应力相关性实验研究(英文)  

Correlation between development of acetabulum in infant rat and mechanical stress force

作　　者：梁庆威[1] 李良满[1] 范广宇[1] 原银栋[1] 

机构地区：[1]中国医科大学附属第一医院骨科,辽宁省沈阳市110001

出　　处：《中国临床康复》2004年第23期4892-4894,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景以往对髋臼发育不良的研究集中在X射线形态学,关于机械应力如何影响髋臼发育及解除机械压力后,髋臼发育不良修复的演变规律研究较少。目的阐明异常机械应力对髋臼发育,尤其对髋臼软骨生长板内软骨细胞增殖的影响,探讨髋臼发育不良的修复方法。设计随机对照的实验研究。地点和对象实验地点为中国医科大学。对象清洁级Wistar大鼠60只,体质量150～200g,均为雌性,周龄为3周,由中国医科大学实验动物部提供。饲养温度18～25℃,湿度40%～70%。干预将3周龄的Wistar大鼠随机数字表法分成3组,每组20只,左侧髋关节为实验侧,右侧为对照侧。A组2周内反复将髋关节手法脱位,而后将其复位;B组膝关节伸直位钢针固定,制作髋臼发育不良的动物模型,2周后拔出钢针;C组持续伸直位固定膝关节。主要观察指标分别于5,7,9,12周龄时进行钼靶软X射线、组织形态学及透射电镜观察。结果A组5周龄时髋臼角比对侧增大约5°,软骨生长板内增殖层软骨细胞极向紊乱,7,9,12周龄时变化轻,两侧无差异;B组5周龄时髋臼角比对侧明显增大,7周龄时柱状细胞层极性紊乱,肥大的细胞层增多,9,12周龄时近正常;C组髋臼角比对侧持续增大,没有任何改善倾向,12周龄时臼缘内翻扁平,无明显柱状细胞排列,细胞核变小,细胞器减少,空泡形成。BACKGROUND: The previous researches on the hypogenesis of acetabulum concentrated on X ray morphology. And there are few researches on the impact of mechanical stress force on the development of acetabulum and the evolution disciplinarian in the restoration of acetabular dysplasia after the removal of mechanical pressure.OBJECTIVE: To clarify the impact of abnormal mechanical stress force on the development of acetabulum, especially on the proliferation of chondrocytes in the chondral growth plate of acetabulum, for the discussion of the restorative methods in acetabular dysplasia.DESIGN:A randomized controlled study was conducted.SETTING and PARTICIPANTS: Setting was in China Medical University. Subjects were 60 females,3 week old Wistar rats in cleanness grade with a body mass from 150 g to 200 g obtained from the Experimental Animal Center of China Medical University, which were fed at 18-25 ℃and in the humidity of 40%to 70%.INTERVENTION:Three week old Wistar rats were randomly allocated into 3 groups according to random number table with 20 rats each.The left hip joint was set as study side and the right side was set as control side.Group A: hit joint was repeated dislocated and repositioned by manipulation within 2 weeks;Group B: the knee joint was straightened for fixation by Kirschner wire to establish the animal model of acetabular dysplasia. Kirschner wire was removed after 2 weeks; Group C: the knee joint was straightened for continuous fixation.MAIN OUTCOME MEASURES: Molybdenum X ray, histomorphology and transmision electron microscope observations were operated at age of 5, 7, 9 and 12 weeks respectively.RESULTS:Group A: at 5 week old, the acetabular angle increased about 5°compared with the other side, and polar derangement of chondrocyte was found in the proliferation layer of the chondral growth plate. The changes at age 7, 9 and 12 weeks were mild with no difference between two sides.Group B:at 5 week old, the acetabular angle significantly increased compared with the other side.At 7 week old,

关 键 词：应力 物理 髋臼/畸形 软骨细胞 

分 类 号：R681.6[医药卫生—骨科学]