44KD受体相关蛋白在实验性高脂血症家兔心肌、血管壁中的表达分析  

EXPRESSION OF THE 44KD RECEPTOR-ASSCIATED PROTEIN(RAP) IN THE CARDIAC MUSCLE AND VASCULAR WALL IN HYPERCHOLESTEROTEMIC

作　　者：胡迎青[1] 汪晓莺[1] 钱绩虎[1] 

机构地区：[1]南通医学院免疫研究室

出　　处：《中国心血管病研究》2004年第9期734-736,共3页Chinese Journal of Cardiovascular Research

摘　　要：目的　探讨 4 4KD受体相关蛋白 (RAP)与脂代谢的关系。方法　制备实验性家兔高脂血症动物模型 ,运用分子生物学技术克隆RAP基因片段 ,制备GST -RAP融合蛋白 ,用单克隆技术制备抗RAP单克隆抗体rapF2 ,用Western -Blot方法及免疫组化法观察高脂血症家兔心肌、血管壁中RAP的表达及分布。结果　与对照组相比 ,高脂血症组心肌、血管壁中RAP表达增加 ,且主要分布于心脏的外膜、冠状动脉内壁与外周血管壁的内、外膜 ,并在6 7～ 94KD间出现特异表达带。结论　血液中过量的脂质及脂质代谢的紊乱可引起血管壁的病理改变并伴随RAP表达增加 。Objective To investigate the expression and distribution of the 44KD Receptor-assciated protein(RAP) in cardiac muscle,coronary and vascular wall in hypercholesterotemic in rabbits. Methods The hypercholesterotemic model was established in rabbits.Fragment of gene(RAP) was gotten by RT-PCR,and the recombinant plasmid(PGEX-RAP) was constructed by inserting the RAP-cDNA into the PGEX. The recombinant plasmid was transfected into DH 5α and expressed the GST-RAP fusion protein by IPTG inducing. The single-clone antibody against RAP was obtained by single-clone technique after the fusion protion purificated.Expression and distribution of RAP was showed with Western-blot and immunohistochemistry technique. Results Compared with control rabbits,the expression of RAP in cardiac muscle,coronary and vascular wall in rabbits of hypercholesterotemic group were increases.The distributi ones of RAP were mainly showed in outer and inner membrane of vascular wall and inner of coronary,cardiac outer membrane.In addition,discovering a exceptional band in 67～94KD. Conclusion The more lipid and inordinate of metabolism in blood would bring on pathology changing in vascular wall,with increasing expression of RAP.The RAP may play a role in the process of metabolism of lipid.The 44KD Receptor-assciated protein may play a role in the process of metabolism of lipid.

关 键 词：受体相关蛋白 高脂血症 基因表达 

分 类 号：R589.2[医药卫生—内分泌]