明胶中α、β和γ三种构象的全分析  

THE FULL ANALYSIS OF DIFFERENT CONFORMATIONS (α, β, γ) IN PHOTOGELATINS

作　　者：陈丽娟[1] 王彤[1] 彭必先[1] 

机构地区：[1]中国科学院感光化学研究所,北京100101

出　　处：《感光科学与光化学》1993年第4期325-334,共10页Photographic Science and Photochemistry

摘　　要：由文献表明:迄今为止,用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)测定明胶分子量分布,只能分出<α,α_1,α_2和β组份,而γ及>γ的组份未能检出。也就是说,大约30万以上的分子量组份未能进入凝胶板内(以下简称“入板”)。这对明胶构象的全分析和对明胶质量的正确判断是一个严重的缺陷。 本文主要探讨和研究γ及>γ组份的入板问题。所采取的方法是:控制丙烯酰胺和甲叉丙烯酰胺(交联剂)的相对含量,选择合适的交联度梯度,使之既适用于α和β组份迁移,也能让γ及>γ的组份入板。此外,在小的交联度和大孔径的情况下,还能符合凝胶板对凝胶强度的基本要求。A careful survey and investigation of the literature references indicate that up to now SDS-PAGE method can be used to characterize the < α, α1, α2, β conformations γ and >γ conformations failed to be realized by this method, it is really a serious fault in full analysis of different conformations of photogelatin and in judgement of the gelatin quality based on conformation components.In general how to handle the contradiction between the crosslinkage degree (hence the pore magnitude) and the physicomechanical property of polyacrylamide gel plate is acknowledged to be the key problem in realizing the full conformation analysis.In present study a series of factors such as the relative concentration of acrylamide and the N,N-methylene-bis acrylamide and the cross-linkage gradient formation were investigated as a function of the migration distance of all conformations. An emphasis was made to observe whether the γ and > γcomponents can enter the polyacrylamide gel plate together with the other conformation components.The experimental data demonstrated that the entire conformation components ranging from < α, α1, α2, β, γ and > γ, without exception, can be separated and characterized with satisfactory resolution by the SDS-PAGE method improved and developed in present study.This improvement and development are of important significance for gelatin routine analysis and controlling the process parameters so as to optimize the correctly relative content of different conformations for a specialized type of gelatin products.

关 键 词：凝胶电泳 构象 照相明胶 

分 类 号：TQ572.2[化学工程—精细化工]