乌贼墨对小鼠脾细胞和巨噬细胞NO生成及IFN-γ分泌水平的影响  

Effects of squid ink on the NO production and IFN-γSecretion by spleen cells and macrophages in mice

作　　者：阎建忠[1] 吕昌龙[1] 李蔚[2] 那立新[1] 刘军[1] 谭颖慧[1] 曹雅明[1] 

机构地区：[1]中国医科大学免疫学教研室,辽宁沈阳110001 [2]沈阳重型集团责任有限公司职工医院,辽宁沈阳110001

出　　处：《中国海洋药物》2004年第4期19-23,共5页Chinese Journal of Marine Drugs

基　　金：由上海澳博海洋生物技术开发有限公司资助。

摘　　要：目的:探讨乌贼墨对脾细胞和巨噬细胞NO生成及IFN-γ分泌的影响。方法:用Griess法和ELISA法分别检测了经乌贼墨灌胃处理后小鼠脾细胞和腹腔巨噬细胞培养上清中NO和IFN-γ的水平的变化。结果:乌贼墨灌胃处理小鼠后的第4、6天脾细胞及腹腔巨噬细胞(M(?))可产生较高水平的NO;脾细胞可产生较高水平的IFN-γ,并且小鼠脾细胞NO的产生水平与IFN-γ产生水平呈正相关(r=0.98);用LPS和L-NMMA(NO抑制剂)分别同乌贼墨灌胃第6d小鼠的脾细胞一起培养,结果显示LPS可协同IFN-γ促NO水平升高。L-NMMA可抑制小鼠脾细胞的NO生成。结论:乌贼墨可促进巨噬细胞NO生成;脾细胞NO的产生与IFN-γ分泌水平呈正相关。Objective To investigate the nitric oxide(NO) and interferon-γ(IFN-γ) levels produced by spleen cells and macrophages in mice treated orally with squid ink. Methods Griess and ELISA assay were used respectively to detect quantitatively NO and IFN-γ in the culture medium of macrophages and spleen cells from mice after the treatment of squid ink. Results NO level produced by peritoneal macrophage and spleen cell from the mice was increased significantly compared to the control and IFN-γ level by the spleen cells was also relatively higher on the day 4 and 6 after the treatment of squid ink. The positive correlation (r=0. 98) was seen between the levels of NO and IFN-γ secreted by the spleen cells. LPS showed the activity promoting the production of NO in coordination with IFN-γ. Conclusion The squid ink is able to promote the production of NO in macrophages. There was a positive correlation between the levels of NO and IFN-γ produced by spleen cells from the treated mice with squid ink.

关 键 词：乌贼墨 一氧化氮 干扰素-Γ 巨噬细胞 脾细胞 

分 类 号：R931.74[医药卫生—生药学]