牛体外成熟卵母细胞孤雌激活的研究  

Parthenogenetic activation of bovine oocytes matured in vitro

作　　者：叶荣[1] 陈学进[2] 郭宪[2] 杨利国[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095 [2]上海第二医科大学发育生物学研究中心,上海200025

出　　处：《南京农业大学学报》2004年第3期133-135,共3页Journal of Nanjing Agricultural University

基　　金：上海市科技兴农重点攻关项目((2 0 0 2 )第 112号 )

摘　　要：比较 3种化学激活方法和 4种电化学激活方法对牛体外成熟卵母细胞进行孤雌激活的效果。结果显示 ,单独用离子霉素和用离子霉素 + 6 DMAP +CB或 +CHX +CB激活牛卵母细胞 ,两者之间差异极显著 (P <0 0 1) ;用不同强度电脉冲 + 6 DMAP +CB激活牛卵母细胞 ,各组间卵裂率差异均不显著 (P >0 0 5 )。结果表明 ,虽然电化学激活方法最高可获得 82 9%的卵裂率 ,比化学激活方法中最好的高 4 8% ,但后者不需电融合仪 ,操作相对简便。Comparisons on parthenogenetic activation were made among two electrochemical and four chemical methods for bovine oocytes matured in vitro .The results indicated that the cleavage rate of oocytes was 24 4% when the oocytes were only treated with ionomycin,while it significantly increased when the oocytes were treated with ionomycin +6 DMAP+CB and ionomycin +CHX+CB(78 1% vs 75 3%)( P <0 01).However the cleavage rates of oocytes activated by different electric pulses +6 DMAP+CB varied little( P >0 05).These results showed that the chemical methods were more convenient than the electrochemical methods although the former had 4 8% less of cleavage rate than that of the latter.

关 键 词：牛 体外成熟 卵母细胞 孤雌激活 电脉冲 6-二甲基氨基嘌呤 离子霉素 

分 类 号：Q813[生物学—生物工程] S823[农业科学—畜牧学]