离子结合物在二茂铁介体电流型葡萄糖生物传感器中的应用研究  

Studies on Application of Ion Associate to Amperometric Biosensors for Glucose Based on Paste Modified by FeCp_2

作　　者：孙长清[1] 宋文波[1] 高倩[1] 杜文媛 许宏鼎[1] 

机构地区：[1]吉林大学化学系,长春130023

出　　处：《高等学校化学学报》1993年第8期1073-1075,共3页Chemical Journal of Chinese Universities

基　　金：吉林大学酶工程开放实验室基金

摘　　要：二茂铁及其衍生物由于氧化还原可逆性好,不溶于水,因此可利用其作介体电流型生物传感器的电子转移介体。但该类传感器的重现性、稳定性差。本文首次提出以含(C_6H_5)_4·(CH_3)_4离子缔合物的碳糊电极为基础电极,以二茂铁(FeCp_2)为电子转移介体制成葡萄糖生物传感器。该传感器重现性、稳定性好,响应迅速,测量准确度高。This paper describes the design and testing of amperometric glucose biosensor in whichelectrical communication between the flavin redox centers of glucose oxidase and a conventionalcarbon paste electrode containing (C6H5).1B · N(CH3)1 has been achieved by using electron-transfer relay system based on ferrocene (FeCp2). Characteristics of the biosensor has been shown by cyclic voltammetry and stationary potential measurements. The biosensor responds rapidly to glucose, with steady-state current responsed achieved in less than 50 s. The response to glucose under N2 saturation showed apparent Michcells-Menten constant, KMapp, 21 mmol/L. The biosensor can be operated in a potential range where the oxidation of common interfering species, such as Vc and uric acid does not occur. Life of the biosensor is 50 days and plasma glucose assays were correlated with spectropllotometric determinations(Y= 10. 04 + 0. 886X, r=0. 99, n=9).

关 键 词：二茂铁 离子缔合物 生物传感器 

分 类 号：Q503[生物学—生物化学]