碳纤维微电极伏安法测定动物脑内5-羟吲哚乙酸、尿酸和高香草酸  

Voltammetric Determination of 5-Hydroxyindol-acetic Acid, Uric Acid and Homovanillic Acid in Animal Brain with Carbon Fiber Electrodes

作　　者：彭图治[1] 王国顺[1] 李惠萍[1] 刘国卿[2] 曹于平[2] 

机构地区：[1]杭州大学化学系,杭州310028 [2]中国药科大学药学系

出　　处：《高等学校化学学报》1993年第10期1374-1376,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金;浙江省自然科学基金

摘　　要：目前,将微电极植入动物脑内,采用伏安法连续监测神经递质已被公认是最有效的在体测定方法。碳纤维电极已成为活体分析中使用的主要电极,为提高其灵敏度和分辨率,电极在使用前必须经过特殊的预处理。An simple electrochemical pretreatment has been used to active carbon fiber electrodes for in vivo determination. In rat brain, there were four well-defined semi-differential voltammet-ric peaks. The peak at 0. 36 V was combined by oxidative current of 5-HIAA and UA, 5-HIAA was about 80% and UA about 20%. The oxidation of HVA formed the peak at 0. 54 V. The concentrations in brain extracellular fluid were determined, 5-HIAA, 3×10-6 mol/L; UA, 9×10-5 mol/L and HVA 8×10-6 mol/L.

关 键 词：碳纤维电极 伏安法 神经递质 尿酸 

分 类 号：R338[医药卫生—人体生理学]