中外11个猪种A-FABP基因微卫星遗传变异的研究  

Genetic Variation of A-FABP Microsatellites in Eleven Pig Breeds

作　　者：李桢[1] 储明星[2] 曹红鹤[2] 李宏滨[2] 马月辉[2] 郑友民[2] 周忠孝[3] 

机构地区：[1]莱阳农学院动物科学系,莱阳265200 [2]中国农业科学院畜牧研究所,北京100094 [3]山西农业大学动物科技学院,太谷030801

出　　处：《遗传》2004年第4期473-477,共5页Hereditas（Beijing)

基　　金：国家重点基础研究发展规划项目(973项目)资助(No.TG2000016104)~~

摘　　要：利用微卫星技术对五指山猪、沂蒙黑猪、汉江黑猪、莱芜猪、北京黑猪、民猪、成华猪、内江猪、二花脸猪、巴马香猪和大白猪11个猪种共420头猪的脂肪细胞脂肪酸结合蛋白基因内含子的微卫星序列的遗传变异进行了研究。研究表明:(1)中国猪种在A FABP基因微卫星基因座上有极高的多态性,五指山猪的PIC最高(PIC=0 7904)并检测到11个等位基因,但北京黑猪仅检测到两个等位基因。(2)本实验中,只有北京黑猪、民猪、巴马香猪和大白猪A FABP基因微卫星在群体中的分布达到平衡状态。(3)群体间的基因分化系数分析表明,中国猪种在该微卫星基因座上分化程度平均约为40.83%。The genetic variations of microsatellites in intron 2 of the porcine adipocyte fatty acid-binding protein ((A-FABP) )gene were investigated in 420 pigs including Wuzhishan pig,Yimeng black pig,Hanjiang black pig,Laiwu pig,Beijing black pig,Min pig,Chenghua pig,Neijiang pig,Erhualian pig,Bama xiang pig and Large White pig.The results suggested as follows:(i)PIC of the Wuzhishan pig breed is the highest(0.7904) and 11 alleles were detected. Compared with Large White pig,Chinese pig breeds showed a great polymorphism of A-FABP microsatellites except Beijing black pig in which only 2 alleles were detected.(ii)Only Min pig, Bama xiang pig, Beijing black pig and Large White pig were in Hardy-Weinberg equilibrium. (iii)The analysis of genetic differentiation showed that the average value of the A-FABP gene differentiation of 10 Chinese pig breeds is about 40.83%.

关 键 词：猪 A-FABP基因 微卫星 分化 

分 类 号：S828.2[农业科学—畜牧学]